Biomarkers Of Clinical Effect In Bipolar Depression Patients Based On Metabolomics

Objective: 1.To find the differential metabolites and related metabolic pathways in plasma of patients with bipolar depression before and after treatment.2.To explore the metabolic markers related to the efficacy of bipolar depression.Method:Patients with bipolar depression and healthy controls who met the inclusion criteria were collected.All patients received standard treatment for 8 weeks.General data,clinical symptoms,blood samples were collected for metabolomic testing.Orthogonal partial least-squares discriminant analysis(OPLS-DA)and variance analysis showed that those with VIP > 1 and P < 0.05 were differential metabolites,and the related metabolic pathways were determined.Result: 1.There was no significant difference in gender,age,BMI and marital status between BD group and control group.2.After treatment,the scores of HAMD,HAMA and CGI scales were significantly decreased,the difference was statistically significant,and the treatment was effective.3.There were seven different metabolites between the patients and the control group.The level of lactic acid increased significantly,while the levels of choline,alpha-glucose,beta-glucose,glycine,N-acetyl glycoprotein and trimethylamine oxide decreased significantly.After treatment,lactic acid,choline,alpha-glucose returned to normal levels,beta-glucose,N-acetyl glycoprotein,trimethylamine oxide levels were reversed to a certain extent,glycine remained unchanged,involving metabolic pathways such as pyruvate metabolism,glycolysis or gluconeogenesis,glycerophospholipid metabolism,starch and sucrose metabolism,pentose phosphate pathway,alanine metabolism,galactose metabolism,glycine,serine and threonine metabolism,aminoglycose and nucleotide glycometabolism.Conclusion:There were seven different metabolites between the patients and the control group.After treatment,six metabolites showed different degrees of regression.Lactic acid,choline and alpha-glucose returned to normal.The related metabolic pathways involved energy,lipid and amino acid metabolism.Therefore,lactic acid,choline and alpha-glucose may be related markers for the efficacy of bipolar depression patients,which can be used to evaluate the clinical effect.