Screening Of Biomarkers For Diagnosis Of Bipolar Depression Based On Fecal Metabolomics

Objective:In this study,differential metabolites in the feces of patients with bipolar depression were screened based on Proton nuclear magnetic resonance(~1H-NMR)metabolomics analysis,to explore their potential as biomarkers for the diagnosis of bipolar depression,and to analyze the possible pathological mechanisms of patients with bipolar depression according to these differential metabolites and involved metabolic types.Methods:1.Subject recruitment:41 patients with bipolar depression and 26 normal controls meeting the inclusion criteria were collected,and general demographic data and clinical symptomatology data were recorded;2.Fecal specimen collection:the fecal specimen meeting the quality requirements should be collected according to the agreed time and conditions with the subject and stored in a-80?refrigerator;3.Spectrum collection and data analysis:nuclear magnetic resonance(NMR)technology was used to collect the metabolic spectrum of feces of the subjects,and the collected data were analyzed by OPLS-DA analysis and independent sample T test to screen the differential metabolites of patients with bipolar depression.Results:1.General information:there was no statistically significant difference in gender,age and body mass index between the case group and the control group,and there were significant symptoms of depression and anxiety in the patient group.2.Fecal differential metabolites:six differential metabolites were screened from the feces of the bipolar group and the normal group,among which glycerol choline phosphoric acid and lactic acid increased in the bipolar group,while butyric acid,adenine,phenylalanine and arginine decreased in the bipolar group.Conclusion:In this study,six differential metabolites were screened out from the feces of bipolar depression and normal people based on ~1H-NMR metabolomics.They are respectively related to energy metabolism,lipid metabolism,amino acid metabolism,purine metabolism and specific intestinal flora metabolism.The specificity and sensitivity of these differential metabolites as biomarkers for bipolar depression diagnosis need to be further improved in the future Repeat the study with large samples.Fecal metabolites are closely related to intestinal microflora.In the future,we can simultaneously detect intestinal microflora,fecal metabolites,blood and urine metabolites of the patients,which can be used as a more effective research method to explore the pathological mechanism of bipolar disorder.