The Role Of Folic Acid-induced EMT In Cervical Cancer Cells

The incidence of cervical cancer is the fourth highest among female malignant tumors,which seriously endangers women's life and health.Although the screening method of cervical cancer can significantly reduce the incidence and mortality of cervical cancer,cervical cancer screening can only find some cervical precancerous lesions as early as possible,can not screen all cervical lesions,and can not be screened.High-risk groups of risk factors.Therefore,to find the relevant factors affecting cervical lesions and further study the molecular mechanism of cervical cancer,can avoid the detection of the corresponding benign lesions and unnecessary treatment,and can provide a theoretical basis for the proposed new preventive measures for cervical cancer.Folic acid is a water-soluble B vitamin that participates in the synthesis of DNA and can regulate gene expression by affecting epigenetic modifications.Folic acid has been found to play an important role in the development and prevention of a variety of malignancies.These studies consistently suggest that blood measurements of folate intake(dietary and dietary supplements)or folic acid are inversely associated with these malignancies.Epithelial-mesenchymal transition(EMT)can cause organ fibrosis through various mechanisms and plays an important role in tumorigenesis and development.EMT confers migration and invasion properties to cervical cancer cells,induces mesenchymal stem cell characteristics,prevents apoptosis and senescence,and escapes immune responses.In this experiment,the expression of EMT-related protein E-cadherin and vimentin in cervical cancer Siha cells without folic acid intervention and folic acid intervention was analyzed by using different concentrations of folic acid to interfere with cervical cancer Siha cells.Folic acid and EMT were analyzed.And the relationship between cervical lesions provides a reliable basis for the prevention and control of cervical cancer.Objective: To investigate whether folic acid can affect the expression of EMT cadherin and vimentin in cervical cancer Siha cells,and whether folic acid participates in cervical epithelial-mesenchymal transition,which may affect the growth,invasion and metastasis of cervical cancer cells.Methods: 1.Cervical cancer Siha cells were cultured and treated with different concentrations of folic acid;2.MTT assay was used to detect the proliferation rate of Siha cells containing folic acid in culture medium and the blank control group without group folic acid intervention,ie group A Cell proliferation rate;3.The scratch test showed that the transfer ability of Siha cells containing folic acid in the medium was lower than that of the blank control group without folic acid,and the transfer ability of Siha cells gradually decreased with the increase of folic acid concentration.4.Transwell invasion assay was used to detect the migration ability of Siha cells containing folic acid in culture medium and the migration ability of Siha cells in blank control group;5.Western Blotting method was used to detect the waveform of Siha cells containing folic acid in culture medium.The expression levels of protein,E-cadherin and Siha cell vimentin and E-cadherin in the blank control group without folic acid intervention.6.The statistical methods were all performed by SPSS22.0 software.The differences between groups were analyzed by one-way analysis of variance.All the measured data were in normal distribution with mean ± standard deviation(X ± S),P<0.05.The difference is statistically significant.Results:1.The MTT assay showed that the proliferation rate of Siha cells containing folic acid in the medium was lower than that of the blank control group without folic acid,and the rate of Siha cell proliferation gradually decreased with the increase of folic acid concentration.2.The scratch test showed that the transfer ability of Siha cells containing folic acid in the medium was lower than that of the blank control group without folic acid,and the transfer ability of Siha cells gradually decreased with the increase of folic acid concentration.3.The Transwell invasion assay showed that the migration ability of Siha cells containing folic acid in the medium was lower than that of the blank control group without folic acid intervention,and the migration ability of Siha cells gradually decreased with the increase of folic acid concentration.4.The results of Western Blotting showed that the expression of vimentin in Siha cells containing folic acid was lower than that in the blank control group without folic acid intervention,and the expression of vimentin in Siha cells decreased with the increase of folic acid concentration.The expression of E-cadherin in Siha cells containing folic acid in the medium was higher than that in the blank control group without folic acid intervention,and the expression of E-cadherin in Siha cells increased with the increase of folic acid concentration.The difference was statistically significant(P < 0.01).Conclusion:1.Folic acid has an inhibitory effect on the proliferation and invasion of cervical cancer cells.2.Folic acid can promote the expression of E-cadherin in cervical cancer Siha cells,inhibit the expression of vimentin to inhibit epithelial-mesenchymal transition(EMT)in cervical cancer cells,thereby inhibiting the occurrence and development of cervical cancer.