1.interlukin 17A Facilitates Osteoclast Differentiation And Bone Resorption Via The Activation Of Autophagy 2.the Combined Treatment Of Non-carious Cervical Lesion With Gingival Recession

Objective:Interleukin 17A(IL-17A)exerts pleiotropic effects on periodontitis,partially through exaggeration in the alveolar bone loss.Osteoclasts are the main culprits that gobble up alveolar bone.However,studies describing the correlation between IL-17A and osteoclast are not conclusive.Previously,autophagy was shown to be involved in osteoclast differentiation and bone resorption.However,the role of autophagy in IL-17A-mediated osteoclast formation is yet unclarified.Methods:Bone marrow macrophages(BMMs)were treated with or without IL-17A.3-methyladenine(3-MA)was applied to inhibit autophagy.Osteoclast formation was detected by tartrate-resistant acid phosphatase(TRAP)staining,immunofluorescence,and scanning electron microscope.The effects of IL-17A on osteoclast-specific genes and autophagy-related genes during the osteoclast differentiation were examined by real-time quantitative polymerase chain reaction and Western blot analysis.Autophagosomes were observed by transmission electron microscope.Hematoxylin-eosin(H&E),TRAP staining and immunohistochemical staining were adopted to assess alveolar bone destruction,the number of osteoclasts and the level of LC3,respectively in rat periodontitis model.Results:IL-17A stimulated the osteoclast differentiation and bone resorption of BMMs with an increase in the number of TRAP+multinucleated cells,the formation of actin rings and the area of bone resorption pit,accompanied by an increase in the mRNA expression of osteoclast-specific genes.Furthermore,IL-17A increased the levels of autophagy-related genes and proteins as well as the number of autophagosomes.After inhibition of autophagy with 3-MA attenuated the IL-17A-mediated osteoclastogenesis and bone resorption.In addition,there was an increase in the number of osteoclasts and level of LC3 and alveolar bone resorption with the IL-17A treatment in the periodontitis model rats.Conclusion:IL-17A facilitates osteoclast differentiation and alveolar bone resorption in vitro and in vivo,and autophagy is involved in IL-17A mediated osteoclast differentiation.Objective:The non-carious cervical lesion with gingival recession involves soft and hard tissue,which leads to complicated therapy in clinical.In this paper,3 patients were included and treated with periodontal plastic surgery with or without restoration.The clinical outcomes were recorded to evaluate the root coverage efficacy.Methods:A total of 3 patients with non-carious cervical lesion combined with gingival recession were treated with periodontal plastic surgeries afterwards.Clinical outcomes were collected and analyzd.Results:Patients reported no discomfort after therapy.Gingival margin level remained stable,ahd the width and thickness of keratinized gingiva increased after therapy.Patients were satisfied with the postoperative results.Conclusion:Periodontal plastic surgery combined with or without restoration could result in a good effect on non-carious cervical lesion with gingival recession.