Experimental Study Of Osteoclast Bone Resorption Supernatants On Protein Expression Of OPG And RANKL In MC3T3-E1Cells

Objective:To investigate the effects of osteoclast bone resorption supematants on the expression of two important regulators of bone remodeling, osteoprotegerin (OPG) and ligand of receptor activator of NF-kB RANKLMethods:The mouse MC3T3-E1cell was cultured with supematants from the osteoclast bone absorbent model in vitro and levels of OPG and RANKL were quantified by Confocal Laser Scanning Microscopy And Imnmnofluorescence Technique.Results:We found that osteoclast bone resorption supematants signitificantly increased OPG levels on day7(P<0.01), RANKL in contrast, was reduced in osteoblasts on day7and day10(P<0.01, P<0.05); In addition, OPG/RANKL ratio appears higher on day7and day10(P<0.05, P<0.05).Conclusions:Osteoclast bone absorbent supernatants might affect the levels of OPG and RANKL by regulating of the differentiation and activity of osteoblast-like cell. Osteocytes are thought to play a principal role in maintenance of bone homeostasis. Background:ingival overgrowth is an increasingly undesired side-effect in patients taking calcium channel blockers. It may cause functional, phonetic, and aesthetic disfigurement, and more importantly, may affect the psycho-social development and quality of life. The management of drug induced gingival overgrowth is a significant challenge for the periodontist and physician.Change in medication should be considered for those patients where the new medication is more reasonabe and economical through the cost-effectiveness methods of pharmacoeconomics. Systemical periodontal therapy has the potential to be a kind of effective treatment mode for drug induced gingival overgrowth recommended in clinic.Objective:To evaluate the clinical effect of systemical periodontal therapy on gingival overgrowth induced by calcium channel blocker(CCBs)without changing or withdrawing the drug.Methods:A total of4patients were enrolled and underwent systemical periodontal therapy including Initial periodontal therapy, periodontal surgery, and periodontal maintenance. No change in medication happened in the process. The bleeding index calculus index, debris index, probing depth and gingival overgrowth index were scored. Periodontal indices and X-ray analysis were evaluated before and after treatment. Results:Systemical periodontal therapy may successfully decrease the severity of drug-induced gingival hyperplasia, reduce the extent of plaque-induced gingival inflammation.All the gingival appeared well vascularized, aesthetically satisfactory, with6to10months follow-up, no one relapsed.Conclusions:Systemical periodontal therapy with follow-up is an essential part of the treatment protocol. Wherever possible this management strategy should be adopted first.However, additional effort is needed from the patients.Initiation periodontal therapy may reduce the rate of recurrence...