The Effect Of Ancient Han Health-keeping Extract On The The Expression Level Of P62 And TGF-?1 In Asthmatic Rats With Deficiency Of Kidney-yang

Objective: To investigate the Effect of ancient han health-keeping extract on the P62?TGF-?1 in asthmatic rats with deficiency of kidneyyang?Methods: 30 healthy male wistar rats aged 4 weeks,weight of(120 ±10)g,were randomly divided into 6 groups(n=5 per group): normal control group(A group),asthma group(B group),asthma of kidney-yang deficiency group(C group),Ancient Han Healtn Essence low,medium and high group(D?E?F group).Cortisol?AL(OH)and OVA was used to establish the deficiency of kidney-yang asthmatic mice model,and the left lung tissues of all mice were treated by HE after the last excitation in 24 h.The airway remodeling of Wat/Pbm?Wai/Pbm and Wam/Pbm were measured by image analysis system.The right lung tissues were detected b by QPCR to investigate the expression level of TGF-?1 mRNA,and by Western blotting to detect the expression levels of P62 proteins.Results:1.HE:The results showed that the bronchial structure of group A was normal,the airway epithelium was intact,and there was no obvious infiltration of inflammatory cells.In group B and C,a large number of inflammatory cells were infiltrated around the bronchial mucosa and bronchial wall,and the bronchial wall was thickened and the mucosa was hyperemia and swelling.Smooth muscle thickened and basement membrane exposed.The pathological changes in group C were more severe than those in group B,and those in the treatment group were significantly less than those in group C,No significant differences were found between Group D?E and Group F(P < 0.05).2.Western blotting: The P62 and protein expression levels in group A were higher than other group(P<0.05),The expression levels of P62 and protein in Group C were significantly less than those in Group B(P<0.05),The treated groups were significantly higher than Group C(P<0.05).No significant differences were found between the expression levels of P62 in Group D?E and Group F(P<0.05).3.qPCR:The TGF-?1 mRNA and protein expression levels in group A were less than other group(P<0.05),TGF-?1 mRNA and protein in group A are in the minimum amount,The expression levels of TGF-?1 mRNA and protein in Group C were significantly higher than those in Group B(P<0.05),The treated groups were significantly less than Group C(P < 0.05).No significant differences were found between TGF-?1 mRNA levels in Group D?E and Group F(P<0.05).4.Image analysis system:In addition to the blank control group,Wat/Pbm?Wai/Pbm and Wam/Pbm was significantly higher than that of A group(P < 0.05).The Wat/Pbm?Wai/Pbm and Wam/Pbm of model control group was significantly higher than that of control group(P <0.05).C group was higher than that of B group(P <0.05).but there was no significant difference in Wat/Pbm?Wai/Pbm and Wam/Pbm between the three groups(P < 0.05).Conclusion:1.The expression of p62 protein was down-regulated and the expres-sion of TGF-? 1 was up-regulated in asthma rats with kidney-yang defic-iency.2.ancient han health-keeping has passive effect on airway remodelin-g of asthma,it maybe regulate the expression of P62 and TGF-?1.