Effect Of LINC01510 On Proliferation And Apoptosis Of Gastric Cancer Cells

Objective: To observe the expression of LINC01510 in gastric cancer cells,to explore the effect of overexpression of LINC01510 on the proliferation and apoptosis of gastric cancer cells,and provide a new therapeutic target for gastric cancer.Methods: The GEPIA database were searched to analyze the expression level of LINC01510 in gastric cancer tissues,and qRT-PCR was used to verify the expression of LINC01510 in various gastric cancer cell lines(AGS,BGC823,MGC803,SGC7901).Low-expression gastric cancer cell lines(SGC7901,AGS)were selected as the research object.The LINC01510 overexpression gastric cancer cell model was constructed by liposome transfection of pCDNA3.1-LINC01510 plasmid.The experimental groups were as follows:LINC01510 plasmid group,empty plasmid group.LINC01510 plasmid group cells were transfected with pCDNA3.1-LINC01510 plasmid,empty plasmid group cells were transfected with pCDNA3.1 plasmid,and qRT-PCR was performed to verify the transfection efficiency.MTT assay was used to observe the effect of overexpression of LINC01510 on priliferation of gastric cancer.TUNEL assay was used to detect apoptosis.The expression level of apoptosis protein Bax was detected by Western blot.Results: 1.GEPIA database analysis indicated that LINC01510 was lowly expressed in gastric cancer tissues;Results of qRT-PCR assay showed that the expression level of LINC01510 was down-regulated in gastric cancer cell lines(AGS,BGC823,MGC803,SGC7901)compared with normal gastric epithelial cells GES-1.The difference in expression between cell lines SGC7901 and AGS was the most significant.2.Construction of LINC01510 overexpression gastric cancer cell model,and the expression efficiency of transfection was detected by qRT-PCR.Compared with the empty plasmid group,the expression of LINC01510 in LINC01510 plasmid group was significantly increased.This showed that the overexpression of LINC01510 model was successfully built.3.Compared with the empty plasmid group,the OD value in LINC01510 plasmid group was significantly decreased at 0 h,24 h,48 h and 72 h in the MTT assay.4.The results of TUNEL test showed that a large green fluorescent expression was observed in the LINC01510 plasmid group conpared to empty plasmid group,which demonstrated that a large number of gastric cancer cells undergo apoptosis.5.Western blot analysis showed that the expression of Bax protein was significantly up-regulated in the plasmid group of LINC01510 compared to empty plasmid group.Conclusion: 1.LINC01510 is lowly expressed in gastric cancer cells;2.LINC01510 inhibits the proliferation of gastric cancer cells and promote gastric cancer cell apoptosis.