The Protective Effect And Mechanism Research Of Curcumin On Radiation-induced Injury Of Human Gastric Epithelial Cells

Objective: To investigate the effects of curcumin on the radiationinduced injury of human gastric mucosal epithelial cells(GES-1),and explore the mechanism of anti-oxidative damage and anti-apoptotic pathway,and provide new possible treatment ideas for the radiation damage in the stomach.Methods: The experiment was divided into four groups,the control,the curcumin groups,the irradiation model group,and the irradiation + curcumin groups.MTT method was used to analyze the viability of GES-1 cells in the irradiated groups.The doses of X-ray irradiation were 1,2,4,and 8 Gy,and the observation time after irradiation was limited to 6,12,24,48 h.A group's irradiation dose and observation time after irradiation when there is an obvious change in cell viability are the irradiation dose and observation time of the irradiation model group.MTT method was also used to investigate whether the treatment of curcumin at 2,4,8,and 16 ?M concentration has an effect on the viability of GES-1 cells.Once again,MTT assay was used to detect whether the different concentrations of curcumin have protective effects on radioactively injured GES-1 cells.DCFH-DA method was used to observe the change of ROS elevation rate of radioactively injured GES-1 cells treated with different concentrations of curcumin.MDA-TBA method was used to detect the MDA content changes in GES-1 cells treated with different concentrations of curcumin.Western Blot was used to detect the expression of apoptosis-related proteins,Bcl-2,Bax and Cleaved Caspase-3,in GES-1 cells treated with different concentrations of curcumin.Results:1.The viability of GES-1 cells decreased in a dose-dependent manner,and the viability of GES-1 cells began to decrease significantly at 4 Gy Xray irradiation after 24 h(P<0.05).GES-1 cell viability was not affected by curcumin treatment at concentrations of 2,4,8,and 16 ?M.As the concentration of curcumin increased,the cell viability of the radioactively injured GES-1 cell model increased in a concentration-dependent manner,and when the curcumin concentration was 4 ?M,the cell viability was significantly higher than that of the irradiated model group(P< 0.05),but none of them returned to the level of the blank control group.2.The increase rate of ROS in the irradiation model GES-1 cell under curcumin treatment showed a drug-dependent decrease trend.The change of ROS in the radioactively injured GES-1 cell model treated with curcumin at a concentration of 2 and 4 ?M was not significantly different from that in the irradiation model group(P>0.05).When the curcumin concentration increased to 8 ?M,the ROS increase rate began to differ from the irradiation model group(P<0.05).3.The MDA content of the irradiation + curcumin groups were lower than that of the irradiation model group,and they were statistically significant(P<0.05),and when the curcumin concentration was 16 ?M,The MDA content was close to the level of the control group,and there was no statistical difference(P>0.05).4.The content of Bcl-2 protein in radioactively injured GES-1 cells increased in a curcumin's concentration-dependent manner,and their contents of Bax and Cleaved Caspase-3 decreased in the same way,but all they didn't return to the level of the control group.Conclusions:Curcumin can exert its protective effect on GES-1 cells through antioxidative damage and anti-apoptosis.Some mechanisms are to reduce its ROS and MDA content,up-regulate Bcl-2,and down-regulate Bax and Cleaved Caspase-3 apoptosis-related protein levels.