Curcumin Alleviates The GES-1 Cells Radiation Damage By Increasing Nrf2 Protein

Objective : The normal human gastric mucosal epithelial cells cultured in vitro by γ irradiation,investigate whether curcumin can protect against radiation-induced gastric injury by activating the Nrf2,and provide new treatment ideas for the radiation damage in the stomachMethods: Based on previous studies,the doses of γ-ray irradiation were 4 Gy and the observation time after irradiation was 24 h.1.The control groups and the curcumin(5μΜ,10μM,20μM)groups were detected by MTT and MDA-TBA method,to observe the effect of curcumin on cell proliferation and intracellular MDA concentration.2.MTT and MDA-TBA method was used to detect the control groups,the irradiation groups,the irradiation+curcumin(5μΜ,10μM,20μM)groups,the irradiation+WR-2721 groups.respectively to observe whether curcumin has the effect of protecting cells and the change of intracellular MDA concentration.3.Western Blot was used to detect the expression of Nrf2 proteins in the control groups,the irradiation groups,the irradiation+curcumin(5μΜ,10μM,20μM)groups,observe whether curcumin can activate the Nrf2.4.The protective effect of Nrf2 on GES-1 cells was verified by adding mL-385.Results:1.Within the experimental concentration range,curcumin has no significant effect on GES-1 cells viability and intracellular MDA concentration.2.Within the experimental concentration range,The radiation-damaged GES-1 cells have a concentration-dependent increase in cell viability after curcumin treatment,when the concentration is 20μM,the treatment effect is the same as that of the positive drug(WR-2721)(P<0.01).3.Within the experimental concentration range,The radiation-damaged GES-1 cells have a concentration-dependent decrease in intracellular MDA concentration after curcumin treatment(P<0.01).4.Within the experimental concentration range,The radiation-damaged GES-1 cells have a concentration-dependent increase in intracellular Nrf2 protein expression after curcumin treatment(P<0.01).5.The cells viability were decreased and the MDA was increased when add the Nrf2 inhibitors(mL-385)to irradiation + curcumin groups.But the GES-1 cells viability was still higher than that in the irradiated group when the mL-385 were added,the difference is statistically significant.Conclusions:1.Experiments show that curcumin can protect GES-1 cells by activating Nrf2 to reduce the intracellular oxygen free γ-ray radical.