The Protective Effect Of Curcumin In The Oxidative Stress Induced Ovairan Injury

ObjectiveTo establish a stable mice model of ovarian oxidative stress.To observe the different effect of curcumin on the markers ofoxidative stress, the expression of ovarian P66Shc and theproliferation of cells in rats ovarian tissue after treatment ofcurcumin, thus exploring the pathogenesis of which to offer moreexperimental evidence for the clinical use of cur cumin on theoxidative stress related ovarian diseases.MethodsSelected75Kunming mice with two regular estrus cycles、withoutcoitus and body-matured female Kunming rats as research objects bythe methods of vaginal smear, randomly divided them into five groups,which included control group,0mg/kg Cur,100mg/kg Cur,150mg/kgCur,200mg/kg Cur. All rats were treated daily for21days andexamined by ELISA for the concentration of Reactive Oxygen Species(ROS),Malondialdehyde(MDA),Superoxide dismutase(SOD) and Glut-athione peroxidase (GSH-Px) in ovaries;Western blotting for theexpression of P66Shc. Immunohistochemical for the proliferation ofcells in ovaries.Results1.During the experiment, there was no abnormal of the physicology and psychology conditions of the mouse, curcumin is asafe drug for mouse. The weight of the five groups mouse wereincreased, the percentage of weight growth are respectively asfollows: control group(16.52±9.62),100mg/kg Cur (16.29±4.86),150mg/kg Cur (15.10±5.67),200mg/kg Cur(13.02±4.19),0mg/kg Cur(11.75±5.88),there was no obvious difference between the groups（P>0.05），the index of ovaries are respectively as follows:200mg/kg Cur (1.0±0.24),150mg/kg Cur (0.98±0.2),100mg/kg Cur(0.96±0.22), control group(0.86±0.19),0mg/kg Cur (0.85±0.15),though there were no differences in each group (P>0.05).After theintraperitoneal injection of arsenic sodium for eight times, theconcentration of ROS(11.74±0.65) and MDA(0.32±0.02) in0mg/kgCur were significantly arised (P<0.05) to compared with the controlgroup(10.71±0.91,0.27±0.02,repectively). Other than theSOD(4.51±0.70) and GSH-Px(18.92±1.80) in control group, theconcentration of both(3.96±0.36,17.36±1.63,respectively) wereremarkable reduced in0mg/kg Cur (P<0.05). Besides, the ovariantissue were destroyed in the ovarian tissue slice, we can findlittle complete follicle structure and abundance atretic folliclesin the0mg/kg Cur.2.To evident whether curcumin plays a protective role in thearsenic induced ovarian injury, the ROS, MDA, SOD and GSH-Pxconcentrations in the ovaries were measured by ELISA. To comparewith the0mg/kg Cur, the concentrations of ROS and MDA in the ovarieswere decreased, while the difference was remarkably significant inboth100mg/kg Cur (10.64±1.38,0.28±0.02,repectively),150mg/kgCur(10.73±0.71,0.25±0.03,repectively) and200mg/kg Cur (10.67 ±1.38,0.27±0.04,repectively)(P<0.05). After the treatment ofcurcumin,the concentration of SOD was significantly increased in100mg/kg Cur,150mg/kg Cur and200mg/kg Cur (4.57±0.68,4.49±0.27,4.56±0.25,respectively)(P<0.05).However, the concentrationof GSH-Px(17.98±1.86,18.01±1.20,18.45±1.65) in100mg/kg Cur,150mg/kg Cur and200mg/kg Cur were increased unconspicuous tocompare with the0mg/kg Cur (P>0.05). Besids, there were no obviousdifferences between the100，150，200mg/kg Cur and the control group(P>0.05). In ovarian tissue slice, we found abundant follicles indifferent stage, and the number of atretic follicles became lowerafter the treatment of curcumin.3.To elucidate the mechanism of curcumin on lipid peroxidation,the expression of P66Shc in ovaries were further examined by westernblot. The expression of P66Shc protein was obviously high in theAs group compared with the control group(0.28±0.004,0.23±0.002,respectively); Compared with the0mg/kg Cur, the100mg/kg Cur,150mg/kg Cur and200mg/kg Cur all have a lower expression(0.18±0.001,0.24±0.007,0.20±0.003,respectively)（P<0.05）. Besids,there were no obvious differences between the100，150，200mg/kgcurcumin treated group and the control group (P>0.05).4.The proliferation of granular cells in0mg/kg Cur wasobviously inhibited, to the contrary, the number of granular cellsin100，150，200mg/kg curcumin treated groups were significantlyincreased to compared with0mg/kg Cur (P>0.05).Conclusion1.From the molecule biology,morphological level and proteo-mics,we found that the mice model of ovarian oxidative stress which conducted by intraperitoneal injection of arsenic sodium wassuccessfully made.2.Curcumin can enhance the concentration of SOD, and reduce theconcentration of ROS、MDA, we conclude that curcumin can improvethe antioxidation ability in mouse ovarian, and scavenge freeradicals, from this way,curcumin exihibits good antioxidantcapacity in mouse ovaries.3.Curcumin can reduce the the expression of P66Shc protein inthe ovary, we infer that curcumin plays important role in thegeneration of ROS via regulate the P66Shc.4.Curcumin can promote the proliferation of granular cells inmouse ovaries.