Experimental Study On The Effect Of "Tiaoshenchangqing Sanliujiu" Acupuncture On Awcd Value And Shannon Index Of Intestinal Microflora In Pd With Insomnia Model Rats

Objective:By comparing with western medicine and routine acupuncture,we observed the effect of "tiaoshenchangqing sanliujiu" acupuncture on behavior,intestinal flora activit y and diversit y of PD with insomnia rats,and explored its related mechanism.Method:The model of PD was established by injecting 6-hydrox ydopamine into unilateral striatum and testing the behaviora l changes induced by apomorphine(APO).On the basis of successful PD model,the model of PD with insomnia was established by intraperito neal injection of the modified PCPA suspension.The model was evaluated by changing the general situation of quantit y a nd so on.This study was divided into six groups: normal group,sham-operation group,model group,drug group,routine acupuncture group and mind-regulating and emotion-regulating acupuncture group.Different intervention methods were used to observe the b ehavioral changes of each group at the corresponding time points.Rat feces were collected and anal yzed by Biolog-ECO method.AWCD value of bacterial flora and Shannon finger were detected.Number,and statistical anal ysis of data results.Results:(1)Compared with the normal group,the behavioral indexes of the model group were significantl y different(P < 0.01),and the AWCD value and Shannon index of intestinal flora were significantl y decreased(P < 0.01).(2)Compared with the model group,the behav ioral symptoms of the drug group improved significantl y(P < 0.01),the AWCD value and Shannon index of intestinal flora did not change significantl y(P > 0.05);the behavioral s ymptoms of the two acupuncture groups improved significantl y(P < 0.05),and the AWCD value and Shannon index of intestinal flora increased significantl y(P < 0.05).(3)Compared with the drug group,the effect of behavioral therap y in the regulating mind and relaxing mood acupuncture group was almost the same(P > 0.05),and there were differences in behavioral indexes in the conventional acupuncture group(P < 0.05).The AWCD value and Shannon index of intestinal flora in the two groups were significantl y superior(P < 0.05).(4)Compared with the conventional acupuncture group,not onl y the behavioral improvement was significant(P < 0.05),but also the AWCD value and Shannon index of intestinal flora increased significantl y(P < 0.05).Conclusion:(1)In terms of improving APO-induced rotation behavior,sleep latency,sleep dur ation,diet and water intake of PD with insomnia model rats,the "tiaoshenchangq ing sanliujiu" acupuncture method has almost reached the effect of Western medicine,and is superior to the conventional acupuncture method.(2)The AWCD value and Shannon ind ex of intestinal flora can be significantl y increased by "tiaoshenchangqing sanliujiu" acupuncture and conventional acupuncture,and "tiaoshenchangqing sanliujiu" acupuncture is superior to conventional acupuncture.Western medicine has no obvious influenc e on the above indexes.(3)One of the mechanisms of "tiaoshenchangqing sanliujiu" acupuncture may be to improve the activit y and diversity of intestinal flora.