Effects Of Acupuncture On Intestinal Flora Disorders And Expression Of IL-6 And IL-1? In Rats With Focal Cerebral Ischemia

Objective:To observe the changes of lactobacillus,bifidobacterium,escherichia coli and enterococcus in intestinal tract of focal cerebral ischemia rats and the effects of acupuncture on the expression of IL-6 and IL-1? in ischemic penumbra brain tissue and serum,and to explore the mechanism of acupuncture on improving intestinal flora imbalance and immunity in ischemic rats.Methods:60 SPF grade male SD rats were randomly divided into five groups,namely,sham surgery group,model group,acupuncture treatment group,acupuncture treatment group,and rehabilitation treatment group.The model of permanent cerebral ischemia was established by modified middle artery suture.The sham operation group and the model group received no treatment.The acupuncture treatment group received acupoint cluster acupuncture,the rehabilitation treatment group received running table training,and the acupuncture and health treatment group received both acupoint cluster acupuncture and running table training.Fluorescence quantitative PCR was used to compare the number of intestinal microflora related species in each group 7days after surgery.Three days and seven days after the operation,the mNSS score of each group was improved,and the expressions of IL-6 and IL-1? in the ischemic penumbra brain tissue and serum were detected by enzyme-linked immunosorbent assay(ELISA).Results:1.Score of improved neural function defect: mNSS score of the model group increased at each postoperative time point compared with that of the sham group(P<0.05).Compared with the model and group,the scores of the three treatment groups decreased(P <0.05),and that of the acupuncture and moxibustion treatment group and the rehabilitation treatment group decreased(P <0.05)7 days after surgery.2.Changes in the number of related bacteria: 7 days after the operation,compared with the sham operation group,the contents of lactobacillus and bifidobacterium inthe model group decreased,while the contents of e.coli and field coccus increased(P<0.05).Compared with the model,the contents of lactobacillus and bifidobacterium in each treatment group increased,while the contents of e.coli and field coccus decreased(P <0.05).The number of lactobacillus and bifidobacterium in the acupuncture treatment group was higher than that in the acupuncture treatment group and the rehabilitation treatment group,and the number of e.coli and enterococcus was lower than the above two groups(P <0.05).3.Expression of IL-6 and IL-1? in serum and brain tissue: the levels of IL-6 and IL-1? in serum and brain tissue of the model group were significantly increased at each postoperative time point compared with the sham group(P <0.05).Compared with the model group,the levels of IL-6 and IL-1? in serum and brain tissue of each treatment group decreased(P <0.05).7 days after the operation,the levels of IL-6 and IL-1? in the serum and brain tissue of the acupuncture and moxibustion treatment group decreased compared with the acupuncture and moxibustion treatment group and the rehabilitation treatment group(P <0.05).Conclusion:1.Acupuncture can improve the score of nerve function defect in rats with focal cerebral ischemia and promote the recovery of nerve function.2.Acupuncture can up-regulate the contents of lactobacillus and bifidobacterium in the intestinal tract of rats with focal cerebral ischemia,and down-regulate the contents of e.coli and field coccus.3.Acupuncture can down-regulate the content of IL-6 and IL-1? in the ischemic penumbra and serum of rats with focal cerebral ischemia.