| Objective: To explore the molecular mechanism of hippocampal participation in the regulation of gastric motility by electroacupuncture at CV12 and BL21 through observing the effect of electroacupuncture at CV12 and BL21 on gastric motility,the expression of c-fos gene and N-methyl-D-aspartate(NMDA)receptor subunit in hippocampus of functional dyspepsia(FD)rats,to explore the central target and molecular mechanism of hippocampus in regulating the cooperative effect of gastric motility in FD model rats by electroacupuncture at CV12 and BL21.Method: Healthy adult sprague-dawley(SD)rats were randomly divided into the normal group,the model group,the weishu group,the zhongwan group,the zhongwan + weishu group,and the non-jingfei point group(14 rats in each group).In addition to the blank group,the rest of the rats were combined with two classical methods to establish the FD rat model.The corresponding acupoints of weishu group,zhongwan group,zhongwan + weishu group,and non-meridian and non-acupoint group were selected for electroacupuncture intervention respectively.The blank group and the model group did not apply intervention measures,and the model group was grabbed and fixed.The stress sensor was used to record the gastric movement waveform in the gastric antrum of rats,and the expression of proto-oncogene c-fos in the hippocampus of each group was detected by immunohistochemical staining.The expression of rat hippocampal neurotransmitter Glu was detected by colorimetry.Western blot(WB)and immunohistochemical staining were used to detect the expressions of NMDA receptor subunits NR1,NR2 A and NR2 B in the hippocampus of rats in each group.Results: 1.FD model evaluation Before the experiment,all groups of rats were in good mental state,with white and shiny fur,sensitive and active.After angering all the caged rats during the modeling,they began to tear and fight with each other.During the fighting,the rats raised their forelimbs from the ground,confronted each other,and shouted "huff".Loose stools and loose stools were observed in the rats 14 days after the model was made.In addition,they smelled foul inside the cage,felt tired and sluggish,and the fight was weakened.Their hair became dark,yellow and rough,and they were irritable and accompanied with nervousness,anxiety and depression.The gastric motility of rats in the normal group and the model group was detected,and it was found that the gastric contraction amplitude of rats in the model group was significantly lower than that in the normal group,and the difference was statistically significant.No organic lesions of gastrointestinal tissue were found in rats in the anatomical model group,which proved that the model building was successful.2.Effect of electroacupuncture on gastric motility in rats Compared with the normal group,the gastric motility amplitude of the model group decreased(P<0.05).Compared with the model group,the gastric motion amplitude of zhongwan group,weishu group,and zhongwan + weishu group increased(all P<0.05),and there was no significant difference between the non-meridian and non-acupoint groups.Compared with the zhong wan group and wei shu group,the gastric motion amplitude of the zhong wan + wei shu group increased(P<0.05).There was no significant difference in the frequency of gastric motor contraction between the normal group,the model group and the electroacupuncture group.3.The effect of electroacupuncture on the expression of neurotransmitter Glu in hippocampus of rats Compared with the blank group,the expression of neurotransmitter Glu increased in the model group(P<0.05).Compared with the model group,Glu expression of neurotransmitter in zhongwan group,weishu group and zhongwan + weishu group decreased(all P<0.05).4.The effect of electroacupuncture on c-fos expression in hippocampus of rats at wei-yu-ji point Compared with the model group,Fos positive cells were significantly increased in the hippocampus of each group after electroacupuncture except the non-acupoint group(P<0.05).In addition,Fos positive cells in the hippocampus were significantly up-regulated(P<0.05)compared with those in the zhongwan group and the weiyu group.There was no significant difference between zhongwan group and weiyu group.5.The effect of electroacupuncture on the expression of NR1,NR2 A and NR2 B in the hippocampal area of rats Compared with the normal group,the expression of NR1 and NR2 B in the hippocampus of the model group increased(P<0.05),while the expression of NR2 A decreased(P<0.05).Compared with the model group,the expressions of NR1 and NR2 B in the hippocampus of rats in the electroacupuncture zhongwan group,weishu group,and zhongwan + weishu group decreased(P<0.05),while the expressions of NR2 A increased(P<0.05).There was no significant difference between the non-meridian and non-acupoint groups.Compared with the zhongwan group and the weishu group,the expressions of NR1 and NR2 B in the hippocampus of rats in the zhongwan + weishu group were lower(P<0.05),and the expressions of NR2 A were higher(P<0.05).Conclusion: 1.The acupuncture information of weiyu and yaoqing acupoints converged in the hippocampus of the limbic system.2.After the information of weiyu and enlii acupoints was uploaded to the hippocampus,the effect of regulating the NMDA receptor by affecting the content of Glu in the hippocampus was achieved. |
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