Affect On Gastrointestinal Motility Of Weixiangle Prescription To Functional Dyspepsia Rat Model

Objective:To observe the effect of Weixiangle Prescription on gastrointestinal motility in the rat model of functional dyspepsia with compound etiology methods,to investigate the mechanism of Weixiangle Prescription,to provide a scientific basis for clinical application and popularization.Methods:60 SD rats were divided into 6 groups after they were all fed by ordinary SPF forage for 7 days by the principle of randomization: normal group,model group,three treatment groups of Weixiangle Prescription classified by high,medium,and low dosage,Mosapride group,10 per group(n=10).Normal group were fed by SPF ordinary forage,other groups induced animal models of functional dyspepsia modeling rats for 21 days.Then dissecting two rats of each group to observe the color of the stomach and duodenum of rats and if there are any organic change and to verify successful model again.After that,return to normal eating and drinking.Except the normal group and the model group,each treatment group received Weixiangle Prescription or were fed by Mosapride,and the normal group and model control group were fed by medical distilled water with the same volume.Gavage the rats at 3:00 PM every day for 14 consecutive days.24 hours fasting after the last administration,then each group was given nutritional semi-solid paste with a dosage of 0.5m L.30 min later the rats were given intraperitoneal injection with 10% chloral hydrate solution with the dosage of 8m L/kg.Then took blood from abdominal aorta and had centrifugal separation in low temperature.The content of plasma motilin were detected.After the rats were killed,weighed rapidly dissected stomach and calculated the gastric emptying rate.At the same time,quickly removed the small intestine and calculated the small intestine propulsion rate.Finally,took two gastric antrums(0.5cm from the pylorus of the stomach tissue)and cut a small piece of a suitable size of the tissue the full thickness was about 0.5cm × 0.5cm and fixed with 2.5% glutaraldehyde,then LKB?ultrathin microtomed the tissue to observe the ultrastructure of interstitial cells of Cajal of the gastric antrum especially mitochondria by transmission electron microscopy.Results:1.General observation: Before the experiment,each of all the rats was argute,active,had glossy hair and in good condition.After the end of modeling,except the normal group,water and food intake in other groups reduced to some degree.The hair turned yellow,dry and dull,stool turned very smelly and dirty.They became nervous and tended to gather in a corner of the cage.2.After the end of modeling,there were no ulceration or organic change in the stomach and duodenum of rats.3.Comparision of gastric emptying rate and small intestine propulsion rate of rats in each group:(1)Compared with the normal group: other groups were significantly lower than normal group(P<0.05).(2)Compared with the model group: each of Weixiangle Prescription groups and the group Mosapride were significantly higher than the model control group(P<0.01).(3)Compared with the middle dose group:the Mosapride group was no significant difference(P>0.05),the high dose group and the low dose group were significantly lower than the middle dose group(P<0.05).4.Comparision of Motilin of rats in each group:(1)Compared with the normal group: the middle dose group was no significant difference(P>0.05),other groups were significantly lower than the normal group(P<0.01).(2)Compared with the model control group:the Mosapride group and the low dose group were no significant difference(P>0.05),other groups were significantly higher than the model control group(P<0.01).(3)Compared with the middle dose group:the low dose group and the high dose group were significantly lower than the middle dose group(P<0.01),but the low dose group and the high dose group was no significant difference(P>0.05).5.Comparison of the ultrastructure of ICC under the electron microscope visible :the ultrastructure of ICC in model group were significantly changed,the connections with the surrounding cells and smooth muscle were reduced,and most the mitochondria has been swollen.Compared with the model group,the ultrastructure of ICC and the connections with the surrounding cells and smooth muscle in other groups had been significantly improved.Vv and?m of mitochondria in each group was significantly difference(P<0.01).?of mitochondria:(1)Compared with the model group:the Mosapride group was no significant difference(P>0.05),the normal group was significantly higher than the model group(P<0.01).(2)Compared with the normal group:the low dose group and the high dose group were no significant difference(P>0.05).(3)each of Weixiangle Prescription groups was no significant difference(P>0.05).Conclusion:1.The functional dyspepsia rat model of this study is a improvement of classical functional dyspepsia rat model with compound etiology methods,and is able to induce the ideal functional dyspepsia rat model.2.Middle dose group of the Weixiangle Prescription of functional dyspepsia rat model's motilin,gastric emptying rate and small intestine propulsion rate,the ultrastructure of interstitial cells of Cajal of the gastric antrum and mitochondria compared to other groups is better,it shows that the middle dose group of the Weixiangle Prescription can be better to promote the gastrointestinal motility.3.The possible mechanism of the treatment of the Weixiangle Prescription of functional dyspepsia rats is that it recover the gastric antrum's ICC especially mitochondria and produce motilin,enhances gastrointestinal motility,thereby promoted gastric emptying and small intestine propulsion.