| Objectivesl)To investigate Chlamydia trachomatis(CT)infection and genotype distribution in pregnant women.2)To explore the relationship between CT genotype and chorioamnionitis infection and mother to child transmission.MethodsThe cervical sercretion of 3.343 pregnant and lying-in women in Banan district maternal and chila health hospital.3.010 neonatal respiratory secretion with non-pregnant reproductive tract infection(non--pregnant infection)and 16,191 cervical secretions of patients with infertility(infertility group)were collected as control.Real-time polymerase chain reaction(PCR)was used to perform CT-DNA detcetiom on the samples,and the positive result samples were subjected to CT genotyping by multiplex or nested PCR-reverse line point hybridization(PCR-RLB).According to the clinical phenotypes of pregnant women,CT genotype an CT typing results of newborns,the main complication,genotype distribution and mother-to-child transmission of CT infection during pregnancy were determined.The statistical methods was accurate test and chi-square test.Results1.3 pairs of primers PCR amplification detected CT strains,after the agarose gel electrophoresis,a band of 202-235bp DNA was found,while the rest of the genitourinary pathogens were not found in the others,indicating that the three primers had strong specificity for CTtetection.2.The minimum detection concentration of multplex PCR-RLB was 100 fg/L,and the minimum detection concentration of nested PCR-RLB was 1000 fg/L.The sensitivity of multiple PCR-RLB to CT was higher than that of nested PCR-RLB.The sensitivity of nested PCR-RLB genotyping was higher than that of multiplex PCR-RLB.3.The average CT infection rate of pregnant women from 2012 to 2016 was 5.0%,and the highest infection rate was 6.9%in 2014.4.The genotypes of CT in pregnant women were mainly E,F and D,with a the mixed infection rates were 30.3%.The most common mixed infection genotypes were J+K(7.7%),E+J(6.0%)and E+F(4.8%).5.Among the pregnant women,168 were infected with CT,the infection rate was 5.0%(168/343);among the non-pregnant women,421 were positive with CT,the positive rate was 9.0%(421/4,686);among the infertile women,1047 were positive with CT,the positive rate was 6.5%(1 047/16,196).The difference was statistically significant(X2= 49.1,P<0.01).There was no significant difference in the proportion of CT genotypes among the three groups(X2= 8.5,P = 0.60).6.There were 312 cases of chorioamnionitis(9.8%,312/3,175)and 47 cases of chorioamnionitis(28.0%,47/168)in 3,175 pregnant women with negative CT resultsand 168 pregnant women infected with CT,respectively,the difference was statistically significant(X2 = 29.561,P<0.01).7.Of the 3,010 neonates,2579 were vaginal delivery,185(7.2%,185/2.579)were CT positive in neonatal respiratory secretions.431 were cesarean delivery,and no CT positive in neonatal respiratory secretion was found.8.The results of nucleic acid detection in 30 infants and their mothers with Chlamydia trachomatis infection were consistent.The results of genotypingin 30 infants with Chlamydia trachomatis were included their mothers with Chlamydia trachomatis infection genotype.Conclusions1.For CT genotyping,multiplex PCR-RLB is a sensitive and rapid method,nested PCR-RLB is a specific method,and the PCR-RLB is better than PCR-RFLP for detecting CT mixed infection.2.The CT infection rate in pregnant women is 5.0%.The infection rates of single-,double-and multiple-CT genotypes are 60.7%,29.2%and 10.1%,respectively.Types of E,F,D are the dominant single genotypes.3.The incidence of chorioamnionitis increases in pregnant women with CT infections during delivery.4.CT can be vertical transmitted(mother-to-child). |
PDF Full Text Request