Transcriptome Analysis Of The Porket Liver Experimentally Infected With Taenia Asiatica

Objective: The aim of this study is to analyze the expression changes of transcriptome in the porket liver infected with cysticercus of Taenia asiatica.It provides basic data for exploring possible molecular mechanisms of Taenia asiatica interaction with intermediate hosts.Methods: The adult of T.asiatica was collected from Liangmu Township,Duyun City,Guizhou Province.Then the gravid proglottid was dissected and washed repeatedly with physiological saline and centrifuged to collect eggs.Twelve 20-day-old York Shiger hybrid porkets were randomly divided into experimental group and control group.Every porket of the experimental group was intragastrically infected with 150,000 eggs.On the 15 th day and the 75 th day after infection,the porkets were sacrificed for collecting the liver tissue of the experimental group and the control group.RNA sequencing(RNA-seq)was used to detect the differential expression of the mRNA transcriptome in the liver tissue of the experimental group and the control group.Bioinformatics analysis and quantitative real-time PCR(q RT-PCR)verification of differentially expressed genes(DEGs)were performed.Results: Compared with the control group,152 and 558 DEGs were detected in the porket liver on the 15 th day and the 75 th day after infection.DEGs accounted for 0.85% and 3.12% of all mRNA genes tested on the 15 th day and the 75 th day after infection.The results of GO and KEGG showed that these DEGs involve functions such as liver immune response,substance metabolism,fibrosis,and tissue proliferative repair.These DEGs are also involved in MHC antigen processing,presentation,IFN receptor-mediated signal cascade,cytochrome P450,TGF-? signaling pathway.The results of q RT-PCR for some DEGs were consistent with the results of RNA-seq.Conclusion: The parasitism of the cysticercus of T.asiatica caused significant differences in gene expression in the porket liver.Specific DEGs in porket liver are involved in biological processes such as metabolism,immune response,and tissue repair or regeneration.These results might help to elucidate the molecular mechanisms of host-parasite interaction.