Protective Effects Of Plantago Asiatica L. And Eucommia Ulmoides Oliv. Extraction On Liver Injury

The liver is the central station of metabolism in vivo, the important detoxication organ, the important target of drugs, xenobiotics and oxidative toxicant. Liver injury is the starting link and common approach of serious liver disease occurrence, development and finally moving towards the liver function failure. The liver injury being caused by various harmful factors were manifested as hepatic necrosis, fatty liver, cholestasis, liver fibrosis, liver cirrhosis and liver cancer and so on.The effects of compound isolated from Plantago asiatica L. and Eucommia ulmoides Oliv. by oral administration on carbon tetrachloride (CCl4) and thioacetamide (TAA) acute liver injury in mice were examined. Then, antioxidant activity of herbal extracts in vitro, the activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), the content of albumin (ALB), total protein (TP), cholesterol (CHO), total bilirubin (TB), glucose (GLU) and the chang of protein in liver were determinated to clarify the possible protection mechanisms.Taken together, the results indicated:(1) Ethanol extract of Plantago asiatica L. was isolated with petroleum ether, ethyl acetate, n-butyl alcohol. The content of water extract, flavonoids and polyphenol compounds in n-butyl alcohol extract, iridoids compounds in ethyl acetate extract is higher. Scavenging effects on DPPH (1,1-Diphenyl-2-picrylhydrazyl radical2.2-Diphenyl-1-(2,4.6-trinitrophenyl)hydrazyl), ABTS (2.2’-Azinobis-3-ethylben-zthiazoline-6-sulphonate)) radical of n-butyl alcohol extract were the strongest, IC50were1.50,5.32μg/mL separately. Scavenging effects on superoxide anion of ethyl acetate extract was the strongest, IC50were149μg/mL. the inhibition effect on lard peroxidation of ethanol extract was the best. Ethanol extract had significantly suppressed the elevation of ALT. AST and TP activities which cased by CCl4(P<0.01). In TAA liver injury experiment, ethanol extract had significantly suppressed the elevation of ALB and TB activities (P<0.01), ethyl acetate and n-butyl alcohol extracts had significantly suppressed the elevation of ALT and AST activities (P<0.01). water extract had significantly suppressed the elevation of TP activities (P<0.01). The ethyl acetate and n-butyl alcohol extracts had inhibition effect on the chang of liver protein expression caused by CCl4and TAA.(2) Ethanol extract of Eucommia ulmoides Oliv. was isolated with petroleum ether, ethyl acetate, n-butyl alcohol. The content of water extract, flavonoids, polyphenol and iridoids compounds in n-butyl alcohol extract is higher. Scavenging effects on DPPH, O2-radical of ethyl acetate extract were the strongest, IC50were105.2、484μg/mL separately. Scavenging effects onABTS radical of n-butyl alcohol extract was the strongest, IC50were43.27μg/mL. the inhibition effect on lard peroxidation of ethanol extract was the best. Extracts hadn’t significantly protective effect on CCl4(5%) liver injury. In TAA liver injury experiment, ethanol extract had significantly suppressed the elevation of ALB and TB activities (P<0.01). ethyl acetate and n-butyl alcohol extracts had significantly suppressed the elevation of ALT、AST and ALB activities (P<0.01), water extract had significantly suppressed the elevation of ALT and ALB activities (P<0.01). The ethyl acetate and n-butyl alcohol extracts had inhibition effect on the chang of liver protein expression caused by TAA.Conclusion:the ethyl acetate and n-butanol extract of Plantago asiatica L. and Eucommia ulmoides Oliv. had obviously protective effects on TAA acute liver damage, which might be related the effect of anti-oxidation, protection of liver cell membrane and inhibition of liver cell apoptosis.