Identiifcation Of Taenia Asiatica From Yunnan By MPCR And Comparative Analysis Research Of Genetic Polymorphism

Objective1.Using MPCR technology on Baoshan， Nujiang， Dali， Diqing inYunnan about HDP2tapeworm Taenia asiatica in specien for genetic polymorphism,so as to exclude false positive of identification of Taenia saginate asiatica specimen.2.Comparing and analyzing all ways about Taenia research from Ynnan province,find afast and reliable gene sequences of Taenia for genetic polymorphism study.3. Proviedthe basis for the classificaition of Taenia asiatica,while make sure the dominantspecies in various parts of Yunnan.Methods:1.With the standard strain of electrophoresis of Taenia from Baoshan,Dali,Nujiang,Puer, using MPCR technology unified study on genetic polymorphismsof gene sequences for HDP2;Using MPCR technology study on14known specimensof Taenia asiatica genomic DNA as a template from Baoshan, Nujiang, Dali, Diqingof Yunnan and drawing electrophoresis diagram.2. Collection and arrangement of thetapeworm around gene sequence research group study results to determine dominantspecies in various parts of Yunnan;Collect all results about gene sequences fromBaoshan,Nujiang,Dali,Diqing, comparative analysis of the gene mutation rate, bases A+T content, PCR amplification time.at last, analysis by SPSS software and drawmaps,clear the type of tapeworm epidemic in various regions in Yunnan.Results:1.The genetic polymorphism study by the Taenia HDP2gene sequencesshow:the figure of1-14appears at the same trip with the figure of Taenia asiatica.2.The comparative analysis of genetic polymorphism in Taenia according tomtDNA-12S rRNA,mtDNA-ND1,rDNA-ITS1, rDNA-ITS2partial gene sequences: The nucleotide variation of the mtDNA-ND1gene was higher than that of themtDNA-12S rRNA gene; The average A+T content of the mtDNA-ND1gene washigher than that of the mtDNA-12S rRNA gene; The homology difference betweenthe mtDNA-ND1gene was higher than that between the mtDNA-12S rRNA gene.The nucleotide variation of the rDNA-ITS1gene was higher than that of therDNA-ITS2gene; The average A+T content of the rDNA-ITS1gene was higher thanthat of the rDNA-ITS2gene; The homology difference between the rDNA-ITS1genewas higher than that between the rDNA-ITS2gene.3.Taenia saginata exist inChuXiong,PuEr,Xishuangbanna; Taenia saginata and Taenia asiatica have place inBaoshan;there are three types of tapeworm in Dali.Conclusion:1. Taenia asiatica exist in Nujiang,Dali,Baoshan,Diqing of Yunnan.Taenia saginata exist in Chuxiong,Puer,Xishuangbanna; Taenia saginata and Taeniaasiatica have place in Baoshan;there are three types of tapeworm in Dali.2.Themultiplex PCR techniques in taxonomic research as reliable effective and convenientway to apply.3.In the study of genetic polymorphism for Taenia,both mtDNA-ND1and rDNA-ITS1showed more superiority.