Study On The Effective Components Of Hypoglycemic In Juglans Chinensis Leaf Based On Spectrum Activity

It is reported that the number of diabetes patients is gradually increasing every year,the situation is very grim.The leaf of Juglans mandshurica studied in this paper is(Juglans Mandshurica Maxim.,which belongs to the genus Juglans in the family Nutaceae.)leaves It is reported that the roots,bark,leaves,green pericarp and nuts of Juglans mandshurica can be used in the treatment of anti-tumor,antioxidation and hypoglycemic diseases.In recent years,the chemical constituents and pharmacological effects of the bark,root bark,pericarp and fruit of Juglans mandshurica have been studied more and more,but little has been reported on the leaves of Juglans mandshurica.The hypoglycemic active substance and its action mechanism of the leaves of Juglans mandshurica were further determined,and the leaves of Juglans mandshurica were studied in depthObjective:The effective parts,effective components and active mechanism of reducing blood glucose in leaves of Juglans mandshurica were determined based on spectrum dynamics of traditional Chinese medicineMethods:Different solvent extracts from leaves of Liriodendron mandshurica were prepared by system solvent method.The inhibitory activities of a-glucosidase and a-amylase were determined in vitro.The hypoglycemic activities of ethanol extracts from leaves of Liriodendron mandshurica were determined,and the effective parts of leaves of Juglans mandshurica were determined.The potential hypoglycemic components in the leaves of Liriodendron mandshurica were screened by targeted affinity-liquid chromatography(HPLC),and the single activity of the potential active components was determined by the in vitro activity screening model of Liriodendron mandshurica.Molecular interaction mechanism of a-glucosidase inhibitor and a-glucosidase in leaves of Liriodendron juglans based on molecular docking techniqueResults:The activities of a-glucosidase and a-amylase were inhibited by ethyl acetate extract from leaves of Liriodendron mandshurica in vitro,and the activity of a-glucosidase and a-amylase were inhibited in vitro.The maximum inhibitory concentrations(IC50)of 0.014 mg/mL and 0.130 mg/mL,were stronger than those of the positive drug acarbose(IC50=0.044 mg/mL and 0.158 mg/mL);,respectively).Screening the active components of Liriodendron mandshurica leaves which could inhibit a-glucosidase by targeting affinity-liquid mass spectrometry,and screened out 15 kinds of potential inhibitory a-glucosidase components in the leaves of Juglans mandshurica.The active components of glucosidase and the structures of eight compounds were inferred.Six monomers were selected for monomer activity verification.The maximum 50%inhibitory concentrations of kaempferol,quercetin and Gallic acid were lower than those of acarbose,a positive drug,in which the concentration of acarbose was lower than that of acarbose.Valuable as a key active ingredient for further research;The active components which can inhibit a-amylase in the leaves of Liriodendron juglans were screened by targeting affinity-liquid mass spectrometry.Eleven active components with potential inhibitory effect on a-amylase were screened and five of them were speculated.Among them,four monomer compounds were selected for monomer activity test,in which the maximum inhibition of kaempferol was 50%.The concentration of Acarbose is less than that of Acarbose,which is the main a-amylase inhibitor in the leaves of Juglans mandshurica.Based on the molecular docking technique,the inhibitory activity and selectivity index of active monomers were evaluated comprehensively.The results showed that kaempferol was the best compound,quercetin,Gallic acid and quercetin-3-O-?-D-galactoside were the best compounds,followed by quercetin-3-O-?-D-galactoside.They are strong with acaibose.Kaempferol-3-O-?-D-glucopyranoside and quercetin-3-O-?-D-glucopyranoside were worse than acarbose.Conclusion:The extract of ethyl acetate from the leaves of Liriodendron mandshurica should be the effective part of lowering blood sugar in the leaves of Juglans mandshurica by enzyme activity test in vitro.Eight potential a-glucosidase inhibitory components in the ethyl acetate layer of Liriodendron mandshurica were preliminarily deduced by targeting affinity-liquid mass spectrometry.Six potential a-glucosidase inhibitory components were tested in vitro.The results showed that all of the six potential a-glucosidase inhibitory components had inhibitory effect on a-glucosidase,and the inhibitory effects of six potential a-glucosidase inhibitory components on a-glucosidase were investigated in vitro.Moreover,the inhibitory activities of Zhongshan phenol,quercetin and Gallic acid were better than that of acarbose and could be used as effective agents.The inhibitory component of a-glucosidase;Five potential a-amylase inhibitory components in the ethyl acetate layer of Juglans mandshurica leaf were preliminarily deduced by targeting affinity-liquid mass spectrometry,and four of the potential a-amylase inhibitory components were determined in vitro.The results showed that all of the four potential ?-amylase inhibitors inhibited a-amylase,and the inhibitory activity of kaempferol was better than that of acarbose,which could be used as an effective component of a-amylase inhibition.Study on the inhibitory mechanism of the potential a-glucosidase inhibitory components in the leaves of Liriodendron juglans based on molecular docking technique Six components,such as kaempferol,can interact with a-glucosidase.The mechanism of hypoglycemic activity of these compounds can be predicted according to the docking site information.