Effect Of ?-elemene On HUVEC Cells Induced By Conditioned Medium In Lung Cancer Cells Under Radiotherapy

Lung cancer has the highest morbidity and mortality in the world,Radiotherapy is one of the main treatments,It is well known that angiogenesis is an indispensable part of tumor growth and metabolism,Under normal circumstances,The vascular endothelial adjacent to tumor cells sprout to form new blood vessels.Radiation kills cancer cells but also destroys nearby blood vessels agency,New research suggests that cancer cells exposed to hypoxic microenvironment during radiation therapy release factors that rewire blood vessels and allow them to regenerate,Experimental studies have found that inhibiting tumor angiogenesis,growth metabolism and malignant invasion of tumor can be effectively inhibited.?-elemene is derived from the Chinese herbal medicine Curcuma Wenyujin,using effective natural compounds extracted using current medical science and technology,which can increase the sensitivity to radiotherapy.Based on the correlation study of the preliminary effects of the drug,(3-elemene can increase the radiotherapy sensitivity of lung cancer,it has certain intervention effect on DNA damage and repair of apoptosis cycle of lung cancer cells.The experiments uses purified injection,Previous studies have found that(3-elemene can play a role in radiotherapy sensitization by inhibiting angiogenesis and the expression of VEGF in lung cancer tissues.This indicates that the mechanism of radiotherapy sensitization of drug is related to tumor blood vessels,However,it's related effects on HUVEC cells and whether it's effects on tumor cells'secretion of related proteins or factors affecting vascular endothelial cells under normal and radiotherapy conditions have not been reported.Research objectivesThe purpose of this study was to investigate the correlation effect of ?-elemene on HUVEC cells,and to directly observe the correlation effect of drug on A549 cells on HUVEC cells under radiotherapy conditions by simulating the radiotherapy environment of lung cancer in vivo through A549 radiotherapy model established in vitroMethods:Part 1:The inhibition of ?-elemene on HUVEC cells.1.MTT assay was used to determine the inhibitory effect of?-elemene on HUVEC cells at different concentrations and the optimal concentration(IC50)2.The effect of ?-elemene on HUVEC migration ability was detected by cell Wound Healing and Transwell experiments3.The effect of(3-elemene on the ability of HUVEC cells to form tubular structures was determined by tube formation experiments4.Western blot was used to detect the expression of related proteinsPart 2:Effect of ?-elemene combined with radiotherapy on HUVEC cells induced by lung cancer cells.1.MTT assay was used to determine the inhibitory capacity and optimal concentration of?-elemene on A549 cells of lung cancer cell line(IC50)2.Experimental groupings:A549 cells were divided into:Control:normal medium;Irradiation:DOSE RATE 600 6MV 4Gy X radiation;?-elemene:?-elemene+culture medium for 24h;?-elemene and Irradiation:after ?-elemene+culture medium for 24h,irradiated with DOSE RATE 600 6MV 4Gy X radiation?Four groups of supematant were collected,namely the conditioned medium(CM)used in the experiment.,HUVEC was treated for 24h,and transwell experiment was used to detect the effect of A549 cell culture medium on HUVEC migration ability3.Hoechst fluorescence staining was used to detect the apoptosis of HUVEC cells in various conditioned medium.4.Western Blot was used to detect the expression of related proteins in HUVEC cells induced by conditioned mediumResults:Part 11.The IC50 of HUVEC cells treated with ?-elemene or 24h was 84.26 g/ml2.With the increase of drug concentration.the inhibition of ?-elemene on HUVEC cell migration ability gradually increased3.?-elemene inhibited the lumen formation ability of HUVEC cells,which was significantly enhanced with the increase of drug concentration4.Compared with the control group,there was no significant change in the expression of PI3K Akt and erk related to HUVEC cell proliferation.However,both p-erk and p-akt reduced the expression,and the expression of migration proteins mmp-9 and mmp-2 was reduced in concentration dependence.Part 21.?-elemene inhibited the proliferation of A549 cells with an IC50 value of 172.89 g/ml.2.Compared with the control group,The migration ability of HUVEC cells induced by the drug group was inhibited to some extent,The inhibitory effect of HUVEC cell migration ability induced by drug combination and radiation group was more obvious3.Compared with the control group,The effect of the drug group on HUVEC apoptosis was not significant,The effect of drug combination with radiation group on HUVEC cell apoptosis was significant4.Compared with the control group,HIF-1? and VEGF protein expression was significantly inhibited in the drug combined with radiation group,and p-akt caspase-3 and bcl-2 expression levels were decreased in the apoptosis-related proteins AKT p-akt caspase-3 and bcl-2.Conclusion:1.?-elemene can inhibit the proliferation of HUVEC cells,which has a concentration-dependent effect on the migration ability and lumen formation ability of cells,and significantly reduces the the expression level of related proteins2.?-elemene inhibits the proliferation of A549,?-elemene combined with radiotherapy can act on A549 cells and change the tumor microenvironment,HUVEC cell migration was inhibited in tumor microenvironment,HUEVC cell apoptosis was promoted,and related protein expression was inhibited.