Study On The Effect And Mechanism Of Xin Mai Jia In The Treatment Of Primary Hypertension

BackgroundHypertension is a cardiovascular disease that can cause heart,brain,kidney and other organ diseases such as myocardial infarction,cerebral thrombosis and renal failure.Many studies have shown that the renin-angiotensin-aldosterone system(RAAS)is involved in the pathological process of devascularization of hypertension arteries.Angiotensin II(Ang II)-specific receptor AT1 receptor causes vasoconstriction and increased blood volume.Previous experimental studies have found that Xin mai jia(XMJ,A Chinese medicinal prescription that is available in capsule form.)has antioxidant and lipid-lowering effects,which can prevent atherosclerosis and restore blood pressure to normal.However,the mechanism of XMJ in reducing primary hypertension(PH)has not been clarified.This study used XMJ to treat spontaneous hypertensive rats(SHR)and its mechanism.ObjectiveIt was verified by animal experiments and cell experiments:(1)Anti-oxidation of XMJ;(2)Hypolipidemic effect of XMJ;(3)Whether XMJ effectively reduced blood pressure by inhibiting the expression of AT1 receptor.MethodA total of 50 spontaneously hypertensive rats(SHR)were randomised into five groups.A total of 30 Wistar-Kyoto rats were randomised into three groups,comprising the control group.All of the rats were administered medicine through a gastrogavage once a day for 8 weeks.Monitor blood pressure weekly.(1)Detection of serum oxidative index;(2)Detection of vascular tissue endothelin(ET),aldosterone(ALD),angiotensin II(Ang II),renin(RE),calcitonin gene-related peptide(CGRP);(3)Detection of arterial endothelium-dependent relaxation response;(4)Preparation of blood vessels Loop,observe the pathological changes of aorta under microscope by hematoxylin-eosin staining;(5)Detect the expression of AT1 receptor in vascular tissue by immunohistochemical staining;(6)Detection of AT1 receptor expression in human aortic smooth muscle cells(HASMCs)by immunofluorescence;(7)The migration of human umbilical vein endothelial cells(HUVECs)was examined by a scratch test.Result(1)Compared with the SHR group,XMJ significantly reduced blood pressure in rats.(P<0.05)(2)Compared with the SHR group,XMJ significantly increased NO and SOD activity in rat serum,and MDA content decreased significantly.(P<0.05)(3)Compared with the SHR group,XMJ can significantly reduce the levels of ET,ALD,AngII and RE,and increase the levels of CGRP in rat serum.(P<0.05).(4)Compared with the SHR group,XMJ had significantly increased endothelium-dependent relaxation of the common carotid artery in rats(P<0.05).(5)Compared with the SHR group,XMJ effectively improved the damage of the vascular endothelium and significantly repaired the blood vessels(P<0.05).(6)Compared with the SHR group,XMJ can reduce the expression of AT1 receptor in vascular tissues and cells of hypertensive rats(P<0.05).(7)Compared with the SHR group,XMJ can decrease AT1 receptor expression in smooth muscle cells(P<0.05).(8)Compared with the SHR group,XMJ inhibited the migration of HUVECs.Conclusion(1)XMJ can reduce the SHR significantiy.(2)XMJ may reduce blood pressure by inhibiting the expression of AT1 receptor and inhibiting the activity of RAAS.(3)XMJ can inhibit vascular dysfunction caused by hypertension,enhance the antioxidant capacity of vascular endothelium,and alleviate the damage of primary hypertension to vascular endothelium in rats.