The Association Of Proinflammatory Cytokine Genes Polymorphisms With Thyroid Associated Ophthalmopathy

Objective To investigate the association of TNF-α gene-308A/G、IFN-ygene UTR5644T/C、IL-12B-1188T/G polymorphisms with thyroid associated ophthalmopathy(TAO)and disease severity in Han population of China.Methods A total of 189 patients with Graves’ disease without GO,154 patients with TAO,and 152 healthy controls were enrolled.According to EUGOGO classification,the TAO group was divided into 3 subgroups:the mild group with 52 patients,the moderate-tosevere group with 55 patients and the sight-threatening group with 47 patients.Genomic DNA was extracted from peripheral blood using magnetic beads and genotyping was detected with the TaqMan-MGB real time PCR method.Statistical analysis was performed using the Chi-squared test and P-values were corrected by the Bonferroni method.Results Polymorphisms of TNF-α-308A/G、IFN-γ UTR5644T/C、IL-12B-1188T/G existed in the 495 subjects.IL-12B-1188 genotype TT+TG were more common in patients with moderate-to-severe+ sight-threatening TAO group than in patients with GD+mild TAO group(P<0.05,OR=2.29),especially in women’s patients(P<0.05,ORF=3.15).No significant association was found between TNF-α-308A/G、IFN-γUTR5644T/C polymorphisms and TAO.Conclusions There were TNF-α-308A/G、IFN-γ UTR5644T/C、IL-12B-1188T/G polymorphisms in Han population of China.The polymorphisms of IL-12B-1188T/G were associated with TAO and disease severity.IL-12B-1188T/G may be the susceptibility genes of TAO in China Han population.No relationship was found between TNF-α-308A/G、IFN-γ UTR5644T/C polymorphisms and TAO susceptibility.