The Effect About Chaihu-shaoyao Compatibility In Prescription Sinisan On Smad Protein And α-SMA In Liver Tissue Of Immune Hepatic Fibrosis Rats

[Objective]Prepare the rat model of immunological liver fibrosis induced byporcine serumthrough intraperitoneal injection,intragastric the rats with Sini Sandifferent dose group(high,medium,low,prevention)and Chaihu-Shaoyao different dose group(high,medium,low,prevention)and the colchicine treatment group respectively,which compare to a blank control group and pathological model group.Then detect Smad2,Smad3,Smad7 index level of liver tissue,α-SMA level,in order to study the mechanism,preventive effect and dose-effect relationship of Sini San and bupleurum peony in combination with liver fiber.[Method]Using 110 SPF-Wistarrats with weights from 110g~130g,which were dividedcompletely random into 11 groups: blank control group,pathological model group,colchicine treatment Group,Sini Sandifferent dose group(high,medium,low,prevention)and Chaihu-Shaoyao different dose group(high,medium,low,prevention).Except the blank group,intraperitoneal injecting on the other groups twice a week,each time 0.5ml Pig Serum continued to 10 weeks,in order to form an immunological liver fibrosis model.The liver fibrosis model can be formed in the five weeks.IntragastricSiniSan Prevention Group,Chaihu-Shaoyao Prevention Group when the model was prepared,and the other groups with the same amount of saline perfusion,then the treatment group from the sixth week,still the blank group and pathological model group with the same amount of saline irrigation.The dosage of each group is as follows: Colchicine treatment group 0.2mg/kg,Sini San groups: High 1.4g/100 g,Medium 0.7g/100 g,Low 0.35g/100 g,Prevention 0.4g/100g;Chaihu-Shaoyao Groups: High 0.7g/100 g,Medium 0.35g/100 g,Low 0.17g/100 g,Prevention0.2g/100 g.Killingall the rats until the tenth week,then studying on data obtainedby immunohistochemical methods with statistical methods.[Results]The Smad2,Smad3,Smad7,α-SMA indices of hepatic fibrosis ratshad been improved to some extentin each treatment group and Prevention group.Compared topathological group,those indices had significant or extremely significant differences in colchicine Group,Sini San groups(High,medium),Chaihu-Shaoyaogroups(High,prevention),and which had significant differences in Chaihu-Shaoyaogroups(High,prevention),but no statistic meaning in Chaihu-Shaoyaogroups(Medium,Low),the same as in Sini San Low group,Chaihu-Shaoyaogroups(Medium,Low).[Conclusion]Apparent antifibrotic effects in Sini San groups(High,medium),and some effects in Sini San Lowgroup,Chaihu-Shaoyao(High,prevention),and prominent effects in Sini San prevention group.