The Clinical Significance Of Serum GOLPH3 Level In Patients With Colorectal Cancer

Objective: In this study,the serum levels of GOLPH3,CEA and CA19-9 were detected in order to evaluate their diagnostic value,and to explore the relationship between the three indicators and clinicopathological factors in colorectal cancer.Methods: 64 patients diagnosed with colorectal cancer and 50 cases of healthy controls were involved in the study from June 2016 to October 2017 in Second Affiliated Hospital of Fujian Medical University.The patients received the first surgical treatment and colorectal cancer was confirmed by pathological diagnosis after operation.All the venous blood were extracted in fasting and the serums were reserved after centrifugation.The level of serum GOLPH3 was detected by ELISA,and the concentration of serum CEA and CA19-9 by ECLIA.ROC curve was used to evaluate the diagnostic value of serum GOLPH3 level in colorectal cancer,and the correlation between the serum GOLPH3,CEA,CA19-9 level and the clinicopathological factors in colorectal cancer was analysed by SPSS 21.0.Results: 1.The level of serum GOLPH3 in patient with colorectal cancer(6.95±6.55ng/ml)is significantly higher than healthy people(3.38±1.66ng/ml),P<0.01.2.The area under ROC curve of serum GOLPH3 concentration for colorectal cancer diagnosis was 0.698,and the best cut-off value of serum GOLPH3 concentration was 6.30ng/ml,exhibited a 42.2% sensitivity,94% specificity.3.The positive rates of serum GOLPH3,CEA and CA19-9 in patient with colorectal cancer were 42.2%,37.5% and 14.1%,respectively.Compared with CA19-9,there were statistically differences in the positive rates of GOLPH3 and CEA(P<0.01),but there was no significant difference between GOLPH3 and CEA(P>0.05).Combined detection of GOLPH3,CEA and CA19-9 raised the positive rate to 59.4% in colorectalcancer,obviously higher than that of single assay(P<0.05).4.The concentration of serum GOLPH3 at 1 week after operation was lower than before operation in patient with colorectal cancer,but the difference was not statistically significant(P<0.05).5.The level of serum GOLPH3 in patient with colorectal cancer was not associated with age,gender,tumor location,tumor size,tumor morphology,lymph node metastasis and distant metastasis(P>0.05).However,the level of serum GOLPH3 in patient with poorly differentiated tumor are significantly higher than the highly to moderately differentiated,furthermore,the level was correlated with depth of tumor invasion(P<0.05).6.The level of serum CEA in patients with colorectal cancer was not associated with age,gender,tumor location,tumor size,tumor morphology,tumor differentiation,lymph node metastasis and distant metastasis(P>0.05),but highly related to depth of invasion(P<0.05).7.The level of serum CA19-9 in patient with colorectal cancer was not associated with patient’s age,gender,tumor location,tumor size,tumor morphology,tumor differentiation,depth of invasion,lymph node metastasis and distant metastasis(P>0.05).8.The level of serum GOLPH3 in patient with colorectal cancer positively correlated with concentration of CEA,CA19-9 and TNM stage,the correlation index were 0.292,0.266,0.280(P<0.05)respectively.The level of serum CEA positively correlated with TNM stage,the correlation index were 0.339(P<0.01).Conclusion: 1.The level of serum GOLPH3 in patient with colorectal cancer was significantly higher than healthy people,the determination of serum GOLPH3 concentration has a certain value for the diagnosis of colorectal cancer,with a sensitivity of 42.2%,a specificity of 94.0%,and the area under ROC curve was 0.698.2.The concentration of serum GOLPH3 at 1 week after operation was lower than before operation in patient with colorectal cancer,but the difference was not statistically significant.The application of serum GOLPH3 level to evaluate the operative effectneeds further study in colorectal cancer.3.The level of serum GOLPH3 was related with differentiation and depth of invasion in colorectal cancer,the more poorly histologic grade and the deeper the infiltration of tumor,showed the higher serum GOLPH3 level.The level of serum CEA was associated with depth of invasion,the deeper extent of infiltrating,the higher serum CEA level was.4.The level of serum GOLPH3 in colorectal cancer positively correlated with CEA,CA19-9 and TNM stage,and the level of serum CEA positively correlated with TNM stage.Combined detection of GOLPH3,CEA and CA19-9 can serve as a predict marker for invasive ability and prognosis of colorectal cancer.