| Objective:Gastric cancer is one of the most common malignant tumors in China.At present,there are no serum tumor markers with high specificity and sensitivity for gastric cancer patients.The GOLPH3 gene has recently been confirmed as a novel oncogene,the latest research found that the serum GOLPH3 level in some cancer patient was significantly higher than in healthy people.It is overexpressed in gastric cancer tissues and is closely related to the development and prognosis of gastric cancer.In this study,the serum levels of GOLPH3,CEA and CA19-9 were detected in order to evaluate their diagnostic value,and to explore the relationship between the three indicators and clinicopathological factors in gastric cancer.Methods:56 patients diagnosed with gastric cancer and 50 cases of healthy controls were involved in the study from June 2016 to October 2018 in Second Affiliated Hospital of Fujian Medical University.The patients received the first surgical treatment and gastric cancer was confirmed by pathological diagnosis after operation.All the venous blood were extracted in fasting and the serums were reserved after centrifugation.The level of serum GOLPH3 was detected by ELISA,and the concentration of serum CEA and CA19-9 by ECLIA.ROC curve was used to evaluate the diagnostic value of serum GOLPH3 level in gastric cancer,and the correlation between the serum GOLPH3,CEA,CA19-9 level and the clinicopathological factors in gastric cancer was analysed by SPSS 21.0.Results:1.The level of serum GOLPH3 in patient with gastric cancer(6.74±1.68ng/ml)is significantly higher than healthy people(3.38±1.66ng/ml),P<0.01.2.The area under ROC curve of serum GOLPH3 concentration for gastric cancer diagnosis was 0.924,and the best cut-off value of serum GOLPH3 concentration was4.54ng/ml,exhibited a 53.6% sensitivity,94% specificity.3.The positive rates of serum GOLPH3,CEA and CA19-9 in patient with gastric cancer were 53.6%,28.5% and 18.1% respectively.The positive rates of serum GOLPH3 concentration in patients with gastric cancer were significantly higher than those of serum CEA and CA19-9.Combined detection of GOLPH3,CEA and CA19-9raised the positive rate to 72.8% in gastric cancer,obviously higher than that of single assay(P<0.05).4.The concentration of serum GOLPH3 at 1 week after operation was lower than before operation in patient with gastric cancer,but the difference was not statistically significant(P<0.05).However,the concentration of serum GOLPH3 at 1 month after operation was significantly lower than before operation and 1 week after operation,and the difference was statistically significant(P< 0.05).5.The level of serum GOLPH3 was associated with the size of the tumor,histological grade,depth of invasion,lymph node metastasis,distant metastasis,and TNM stage.6.The level of serum CEA in patient with gastric cancer was not associated with age,gender,tumor size,tumor morphology,lymph node metastasis and distant metastasis(P>0.05).However,Serum CEA concentration was positively correlated with depth of tumor invasion and distant metastasis(P<0.05).7.The level of serum CA19-9 in patient with gastric cancer was not associated with patient’s age,gender,tumor location,tumor size,tumor morphology,tumor differentiation,depth of invasion,lymph node metastasis and distant metastasis(P>0.05).8.The level of serum GOLPH3 in patient with gastric cancer positively correlated with concentration of CEA,the correlation index were 0.532(P<0.05).However,there was no correlation between concentration of GOLPH3 and CA19-9.Conclusion:1.The level of serum GOLPH3 in patient with gastric cancer was significantly higher than healthy people,the determination of serum GOLPH3 concentration has a certain value for the diagnosis of gastric cancer,with a sensitivity of 53.6%,a specificity of94.0%,and the area under ROC curve was 0.924.2.The concentration of serum GOLPH3 at 1 week after operation was lower than before operation in patient with gastric cancer,but the difference was not statistically significant.However,the concentration of serum GOLPH3 at 1 month after operation was higher than before operation and 1 week after operation in patient with gastric cancer,the difference was statistically significant.3.The level of serum GOLPH3 was associated with the size of the tumor,histological grade,depth of invasion,lymph node metastasis,distant metastasis,and TNM stage.4.The level of serum GOLPH3 in gastric cancer positively correlated with CEA.5.Combined detection of GOLPH3,CEA and CA19-9 can serve as a marker for judging the invasive ability of gastric cancer and monitoring after operation. |
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