The Expression And Clinical Significance Of GOLPH3 And MTOR In Breast Cancer Tissue

Background:Breast cancer is the most common malignant tumor in women all over the world.In recent years,its incidence has increased.It is very important to detect the new molecular biomarkers of high expression in breast cancer.Genome-wide copy number analyses of human cancers identified a frequent 5p13 amplification in multiple solid tumor types.Integrative analysis of the region identifies a Golgi protein phosphorylation,GOLPH3(also known as GMx33 or GPP34),as a candidate target for amplification.GOLPH3 plays a key role in the process of receptor recovery,glycosylati-on and protein transport from golgi body to plasma membrane,as a highly conserved stromal membrane protein in gene sequences.Recent studies have shown that high expression of GOLPH3 is associated with poor prognosis of many tumors,and can be used as a biological indicator to judge the prognosis of multiple tumors.However,the role of the gene in breast cancer and the relationship between the expression products and the clinical features of breast cancer are less reported.MTOR,the target molecule of rapamycin,is a serine/threonine kinase.It is the central regulatory factor for cell growth,metabolism,proliferation and survival.One research has shown that inhibiting mTOR is an effective strategy for treating cancer,slo-wing the growth of tumors and limiting the spread of cancer.Scott's experimental group analyzed the large-scale chemical genome of yeast,and proposed a hypothesis that GOLPH3 might regulate the homologous compound of TOR(mTOR),leading to the tumor effect of GOLPH.Therefore,the study of GOLPH3 and mTOR expression in breast cancer and its correlation,not only provide theoretical basis for researching mechanism of GOLPH3,but also provide guidance for the diagnosis and prognosis of breast cancer.Objective:1.To clarify the expression of GOLPH3 and m TOR in breast tumor tissues;2.To explore the correlation between the expression level of GOLPH3 and mTOR and the clinicopathological features of breast cancer patients;3.To explore the correlation between the expression of GOLPH3 and m TOR in breast cancer;Methods:A total of 146 filed mammary gland pathological paraffin blocks were collected,which were excised in the second hospital affiliated to Dalian medical university from 12,2013 to 05,2017.They include 100 cases of breast cancer Paraffin blocks,20 cases of benign fibroadenoma Paraffin blocks,and 26 cases of para-cancer normal tissues Paraffin blocks.The clinical information characteristics of paraffin blocks were collected and recorded.The samples of the paraffin samples were taken into slices,and the expression of GOLPH3 and mTOR in breast tumor tissues,fibrous adenoma tissues and adjacent normal tissues were detected by SP immunohistochemist-ry.SPSS statistical software was used to analyze the correlation between the expression of GOLPH3 and mTOR and the relation with clinical pathological features of breast cancer.Results:1.The positive expression rate of GOLPH3 protein are 76.0%(76/100)in breast cancer tissues,40.0%(8/20)in benign fibroadenomas and 42.3.0%(11/26)in normal tissues adjacent to carcinoma.While about mTOR,the positive expression rate are 71.0%(71/100),45.0%(9/20),34.6%(9/26).Allthedifferencesabovewerestatistically significant.2.Expression of GOLPH3 in breast cancer tissu is related with TNM stage(P=0.001),tumor size,lymph node metastasis(P=0.020),the expression of HER2(P=0.020)and the ki-67 state(P=0.019).It was not related to age,ER,PR,vascular metastasis,etc.Expression of mTOR is related with lymph node metastasis(P=0.031),ER(P=0.000),expression of HER2(P=0.002),ki-67 state(P=0.024),while it shows no relations with other factors(P>0.05).3.In breast cancer,the expression of GOLPH3 and mTOR is significantly positive(P=0.021,X~2=5.296).Conclusions:1.GOLPH3 and mTOR show significantly higher expression in most breast cancer tissues than in benign fibroadenoma tissue and para-cancer normal tissue.2.In breast cancer,GOLPH3 is positively correlated with TNM stage?tumor size?lymph node metastasis?the expression of HER2 and the ki-67 state,while mTOR is correlated with lymph node metastasis?ER?expression of HER2 and ki-67 state.3.The carcinogenic mechanism of GOLPH3 may be associated with mTOR,and GOLPH3 may be a potential target for breast cancer diagnosis.