Effects Of Tongdu Huoxue Decoction On IL-1??TNF-??TXB2?6-Keto-PGF1a In Rabbit Model Of CSA Of Kidney Stasis Syndrome

Objective To study the effect of Tongdu Huoxue Decoction on CSA model of rabbit peripheral blood interleukin-1??IL-1??,tumor necrosis factor alpha?TNF-??,thromboxane B₂?TXB₂?,6-keto prostaglandin?6-Keto-PGF1a?the effects of keto prostaglandin,and drug intervention of cervical spondylosis of vertebral artery type other mechanism,to provide experimental basis for CS clinical treatment.Methods a composite type of vertebral artery type of blood stasis syndrome and kidney model of cervical spondylosis rabbits by intramuscular injection of hydrocortisone and sclerosing agent injection combined method,and the establishment of the normal group,model group,celecoxib group,Jingfukang group Tongdu Huoxue Decoction high,low dose group,7 control group,continuous the medicine pathological change 4 weeks.HE observation of cervical muscle;enzyme linked immunosorbent assay?ELISE?IL-1?,TNF-?and plasma TXB2,6-Keto-PGF1a content;immunohistochemical detection of rabbit intervertebral disc tissue IL-1?expression level of TNF-?,TCD;detection of vertebral artery and basilar artery pulsatility index?Pi?and the resistance index?Ri?changes;blood rheology test hematocrit and erythrocyte aggregation index change;ELISE detection model of rabbit serum triiodothyronine three?T3?,four triiodothyronine?T4?content.Resultsthe model group increased serum IL-1?and TNF-?content,decreased plasma TXB₂ content and 6-Keto-PGF1a content of the intervertebral disc tissue IL-1?,increase the expression of TNF-?,Pi Ri of vertebrobasilar artery,elevated hematocrit and erythrocyte aggregation index increased,decreased serum T3 and T4content,compared with the normal groups were statistically significant the difference?P<0.01,P<0.05?.Compared with the model group,the treatment group pathological morphology can be improved in different degrees of rabbit muscle,serum IL-1?and TNF-?content,lower the level of TXB₂ and increase the content of 6-Keto-PGF1a,reduce the IL-1?intervertebral disc tissue,the positive expression of TNF-?,reduce the vertebral basilar artery Pi,Ri,reduced red hematocrit and erythrocyte aggregation index,serum T3 and T4 content,the high group best effect,and the other in each group were significantly higher?P<0.01,P<0.05?.Conclusion Tongdu Huoxue decoction has certain therapeutic effects on the kidney and blood stasis syndrome of vertebral artery type of cervical spondylosis rabbits,and its mechanism may be related to the inhibition of inflammatory factor,regulate vascular factor,improve intervertebral disc tissue inflammatory factors,improve blood circulation,improve the expression of the viscosity of blood and regulating the secretion of thyroid follicular epithelial cells about.