The Effect Of "treatment Of Acupuncture On Back And Limbs" On The Content Of IL-17,MMP-9 And TIMP-1 In The Serum Of RA Model Rats

?Objectives?Observe RA(Rheumatoid arthritis)model rats in acupuncture and moxibustion intervention nakedness quality,the joint swelling improve,serum IL-17,MMP-9 and TIM P-1content expression changes,in order to clarify acupuncture through regulating cytokines,play the role of improving RA symptoms.At the same time,the acupuncture and moxibustion stimulation of the same acupoint combination were observed.To provide scientific laboratory basis for the treatment of rheumatoid arthritis and rheumatoid immunity.?Methods?The SPF male rats,50,weighed and numbered,Removal of polar mass(10),then were quadripartition into blank group,model group,acupuncture group and moxibustion group by the random number table method.Then give them number mark.Except the blank group,each rat was injected the Freund's complete Adjuvant(FCA)on 0.15 ml to turn into rat model of Adjuvant arthritis(Adjuvant arthritis,AA).Visible after 24 h of the model group rats right rear foot plantar markedly swollen,with method of immune to strengthen after 7d,secondary inflammatory reaction after 21 d,show the fore or the contralateral limb ears,tail swelling or inflammatory nodules appear,even in building success.In the blank group,the right posterior foot of the rat was injected with 0.15 ml of 0.9%Nacl solution after alcohol disinfection.Treatment intervention to select the back: “Dazhui”,“Mingmen”;Upper limbs: “Hegu”,“Quchi”;Legs:“Sanyin jiao”,“Yanglingquan”,“Zusanli;Combination of “Treatment of acupuncture on back and limbs”combination of acupoints,acupuncture group was given acupuncture 20 min,retaining needle moxibustion group every distance is 2 cm suspended moxibustion acupoints in 20 min per day,7 days for a course.In totol two course of treatment.Measure the body mass of rats in each group were observed and measured the later 2 weeks.After treatment,By means of ELISA to assaying the contents of IL-17,MMP-9 and TIMP-1 in serum of rats.?Results?(1)Compared with the blank control group.When set the rat modle,model group?acupuncture group,and moxibustion group right foot and ankle swelling of rats,and eat less,hair messy lacklustre,body quality increase slowed(P<0.05),right rear foot metatarsus swelling degree increased(P<0.05).After treatment,the mass growth of the acupuncture group and moxibustion group was small(P<0.01),and the swelling degree of the right hind foot was little.The mass growth of the model group was relatively large(P<0.05),and the swelling degree of the right hind foot was increased(P<0.05).The contents of IL-17,MMP-9 and TIMP-1 in the serum of the three groups were all increased(P<0.05).(2)Compared with the model group.After modeling,there was no statistical difference between with acupuncture group,moxibustion group quality,and right posterior foot of the foot.(P>0.05).Intervention in 1 week,2 weeks after treatment,acupuncture and moxibustion group body quality growth slowed(P<0.05),right rear foot metatarsus swelling degree were decreased(P < 0.05),serum IL-17,MMP-9 and TIMP-1 content decreased(P<0.05)(3)Compared with the moxibustion group.After treatment,the degree of improvement of the swelling degree of the right posterior foot was comparable to that of moxibustion group,with no statistical difference(P>0.05).The expression of IL-17 in the serum of the acupuncture group was similar to that of moxibustion group,with no statistical difference(P>0.05),while the content of MMP-9 and TIMP-1 reduced significantly,with statistical difference(P<0.05).?Conclusions?(1)In this experiment,acupuncture and moxibustion therapy can improve the joint swelling of rats.the effect is equal.(2)Acupuncture and moxibustion can effectively reduce IL-17 size of the RA model.Expression in rat serum inhibits the differentiation of Th17 cells,inhibits the activation of synovial cells and produces synergistic effects with IL-1 and TNF-?,inhibiting the secretion of MMP and other proinflammatory factors.This prevents bone and cartilage destruction.(3)Maintaining MMP-9/TIMP-1 balance is the key to protecting articular cartilage and bone joint.The treatment can effectively make the expression of MMP-9 and TIMP-1 in serum reduce,and can control the occurrence of synovial inflame mation.The effect of acupuncture is better than moxibustion.