Studies On The Antidepressant-like And Prokinetic-like Effects And The Underlying Molecular Mechanisms Of The Chemical Constituents Of Fructus Aurantii Magnolia And Hesperidin

Background:Depression is caused by a variety of reasons to persistent low mood as the core symptoms,accompanied by mental retardation,cognitive impairment,volitional activity drops,mental disorder sleep dysfunction and gastrointestinal dysfunction.Clinical antidepressant drugs are mainly 5-HT reuptake inhibitors(SSRI),tricyclic drugs(TCA),monoamine oxidase inhibitors,but there are many side effects.This study is to explore the antidepressant mechanism of traditional Chinese medicine.The chemical constituents of Fructus aurantii Magnolia have the effect of shugan-liqi and xiaopi-chuzhang,and it can ease chest flank pain,belching and sighing,poor appetite,abdominal distention,grief symptoms.Hesperidin is the effective absorption of chemical constituents of Fructus aurantii Magnolia,and they both have the effect on anti-depression and gastrointestinal motility.Objective:The purpose of this study was to investigate the effect and mechanism of anti-depression and gastrointestinal motility of the chemical constituents of Fructus aurantii Magnolia and hesperidin.Methods:First,we used UPLC confirmed the compounds absorbed into blood of the chemical constituents of Fructus aurantii Magnolia.Second,the forced swimming test and gastrointestinal motility test were used to demonstrate the antidepressant-like and prokinetic effect of the chemical constituents of Fructus aurantii Magnolia and hesperidin.Moreovere,hesperidin induced anti-depression similar to Fructus aurantii magnolia via Enzymes of the Kynurenine Pathway and regulation of ghrelin.Results:First,we confirmed hesperidin was one of the effective active ingredient absorbed of the chemical constituents of Fructus aurantii Magnolia.Second,hesperdin induced antidepressant-like effect and prokinetic-like effect similar to the chemical constituents of Fructus aurantii Magnolia.Third,the antidepressant mechanism of Fructus aurantii MagnoliMagnolia.Both of them exert antidepressant-like effect is through altering the function of the gastrointestinal tract and induction of the Kynurenine Pathway.a and hesperidin was achieved by the change of tryptophan and kynurenine metabolic pathway and gastrointestinal function.Conclusion:Hesperidin plays an key role in the pharmacological effect of Fructus aurantii.