Construction Of Fingerprint And Analysis Of Bioactive Ingredients From Hunan Aurantii Fructus

Aurantii fructus is the dried and immature fruit of Citrus aurantium L.and its cultivars.As a traditional Chinese medicine,it has been intensively applied to treat gastrointestinal motility disorder in conventional therapies.However,the detail of basic components,qualitative analysis and key functional compounds of Hunan Aurantii fructus,in particular,are few reported.Therefore,extraction technology,chemical composition,qualitative fingerprint and biological activity of Hunan Aurantii fructus were studied in this thesis.1.Based on the response surface Desirability function method,total flavonoids and antioxidant activity were assigned as indicators,technological extraction of the effective components was investigated.In the optimum process,the concentration of ethanol,extraction temperature and time were 58%,70,and 17 min,respectively.The content of total flavonoids and antioxidant activity were 240.35 mg/g and 59.45%,respectively.2.The ethanol extract and volatile oil of Aurantii fructus were analyzed by UPLC-Q-TOF/MS and GC-MS method.A total of one hundred-four compounds were identified.A sum forty-one compounds has been tentatively identified for the first time in Aurantii fructus.3.A method of simultaneous determination of 12 main effective components in Aurantii fructus was established by high performance liquid chromatography.Based on 8 common characteristic peaks,the HPLC fingerprint and the evaluation system of Aurantii fructus were established in the main producing area of Hunan province.The average content of naringin and hesperidin was more outstanding than the standard of Chinese Pharmacopoeia.4.The potential dopamine D2 receptor antagonist in Aurantii fructus was analyzed by molecular simulation,5 potential D2 receptor antagonists were screened.The molecular mechanism of naringenin to D2 receptor was consistent with the positive control(domperidone and 8NU).The molecular interaction between antagonists and receptors was explained by molecular simulation,and the pharmacological mechanism for treatment of the gastrointestinal motility disorder was illustrated.