Sox4 Affects The Adipogenesis By Wnt Signaling Pathway

Obesity derives from the imbalance between food intake and consumption,leading to hypertrophy and hyperplasia of adipocytes and a series of metabolic disorders such as hyperlipidemia,insulin resistance and type 2 diabetes.These are all based on the commitment and adipogenic regulation of mesenchymal stem cells(MSCs)in the marrow and vascular stroma of adipose tissue,which are divided into two stages:the commitment from mesenchymal stem cells to preadipocytes and terminal differentiation.Adipogenic regulation of MSCs involves two major signaling pathways:BMP signaling pathway and Wnt signaling pathway.Both canonical and non-canonical Wnt pathways play an important role in the commitment of stem cells towards preadipocytes.Wnt5a-mediated non-canonical pathway can promote the commitment of preosteoblasts and inhibit that of preadipocytes.Previously,we have found that Sox4,an important protein downstream of BMP4,is closely related to the commitment of mesenchymal stem cells via Microarray analysis.In this study,we investigated the role of Sox4 in the regulation of adipocyte commitment at the molecular and cellular level,and confirmed that Sox4 can activate the Wnt signaling pathway.Moreover,Wnt signaling pathway is capable of inhibiting adipogenesis,indicating that Sox4 is involved in regulating commitment via Wnt signaling,providing reference for completely revealing the molecular mechanism of commitment and differentiation as well as the role of autophagy pathway in adipose differentiation.