MicroRNA 212 Inhibites The Proliferation,Migration And Invasion Ability Of Hepatoma Cell By Down Regulating SOX4 Gene

Objective: We aim to illuminate the crosstalk between microRNA212(miR-212)and the gene SOX4(sex determining region Y-box 4)in the hepatocellular carcinoma cells and to confirm the functions of mi R-212 in proliferation,migration and invasion.Thus,we hope to provide the potential targeted site and theoretical basis in the clinical treatments.Methods: Real-time PCR assays were respectively used to detect the expressions of mi R-212 and SOX4 mRNA in human hepatocellular carcinoma tissues,and five cell lines.We built a mimic negative control group and miR-212 mimic experimental transfection group.MiR-212 plays an important roles on the proliferation,migration and invasion of hepatocellular carcinoma cells,QGY-7703,and these were respectively detected by CCK8,wound healing assay and Transwell chamber experiment.The relationship between SOX4 and miR-212 was validated by the Dual-Luciferase reporter gene experiment.Results: miR-212 was down-regulated in human hepatocellular carcinoma tissues,compared with adjacent normal tissues(*P<0.05).And compared with the expression of mi R-212 in L-02,the other four hepatocellular carcinoma cell lines,SMMC-7721,Huh,QGY-7703 and HepG2,were deteced less(*P<0.05),and of all,the lowest expression was in QGY-7703 cell lines.It suggests that miR-212 is generally down-regulated in liver cancer.The expression of SOX4 mRNA was up-regulated in the hepatocellular carcinoma tissues(**P<0.01),and higher than that in the L-02 cell lines(*P<0.05).The proliferation of miR-212 mimic transfection group was significantly lower in the CCK8 test than that in the mimic control group(*P<0.05),which indicates overexpression of mi R-212 could inhibit the proliferation of HCC.The migration and invasion of miR-212 mimic transfection group were lower than the control group(*P<0.05),meaning miR-212 could suppress the migration and invasion abilities of hepatoma cells.And with the Dual-Luciferase reporter gene experiment,the luciferase activity of miR-212 mimic + 3'UTR SOX4 group was lower than that of mimic + 3' UTR SOX4 group(*P<0.05),which demonstrates that mi R-212 could regulate the expression of SOX4 directly.GraphPad 5.0 was used in statistics.t-test and analysis of Variance were applied to compare the difference among different groups.And when P<0.05,the diference is statisticly significant.Conclusion: MiR-212 is poorly expressed in hepatocellular carcinoma tissue andcell lines,and the expression of SOX4 is down-regulated by miR-212 directly,and then inhibit the proliferation,migration and invasion of hepatocellular carcinoma through the interaction of them.