Study On Expression Of Long Non Coding RNA RP11-69I8.3 In Acute Leukemia And Its Cinical Significance

Backgroud and ObjectiveAcute leukemia(AL)is a group of malignant clonal disease which originated from hematopoietic stem cells,due to the enhancement of leukemia cell self-renewal,proliferation out of control,inhibition of apoptosis and the proliferation,accumulation of abnormal primitive cells and immature cells(leukemic cells)in the bone marrow,result in normal hematopoietic inhibition and infiltration to other organs and organization.Its occurrence is a complicated pathophysiological process,which is mediated by a variety of factors.The abnormal changes in genomics and protein levels are the hot spots of the present study.Long non coding RNA(lncRNA)is a class of DNA transcripts that more than 200 nucleotides without encoding protein function.LncRNA can participate in the process of cell differentiation,proliferation and apoptosis through various mechanisms such as transcriptional process interference,X chromosome inactivation,genomic imprinting and intra nuclear transport etc.Recently,studies have found that abnormal expression of lncRNA in the bone marrow cells of AL patients.The abnormal expression of lncRNA not only regulates the proliferation of tumor cells,activates the invasion and metastasis of tumor cells,but also induces vascular regeneration,resistance to apoptosis of cancer cells and so on.More,it can lead to the resistant to chemotherapeutic drugs of tumor cells.Our research used the gene chip technology in ealier stage to analysis the expression spectrum of the lncRNAs of acute leukemia and choose lncRNARP11-69I8.3 from the abnormal expressed lncRNAs.Then we used real-time fluorescence quantitative PCR method for further study.The expression of lncRNA RP11-69I8.3 in acute leukemia has not been reported.The purpose of this study was to find lncRNA associated with acute leukemia,to explore the relationship of the abnormal expression of lncRNA with the occurrence and development of acute leukemia.Then analyzed the relationship of the lncRNA with the clinical characteristics of acute leukemia.MethodsCollected 32 cases fresh bone marrow samples which were newly diagnosed acute myelocytic leukemia(AML).There were 17 cases of males and 15 cases of females which median age was 30 years old(14-60).32 cases of newly diagnosed ALL(acute lymphoblastic,leukemia),including 22 cases adult ALL and 10 cases children ALL,26 cases of B-ALL and 6 cases of T-ALL.There were 18 cases of male and 14 cases of female which median age was 24 years old(1-60).The time was from March 2016 to May 2017 in the department of hematology and department of prediatric of the first affiliated hospital of Zhengzhou university.25 cases of complete remission ALL after chemotherapy were traced,including 22 cases of adults ALL and 3 cases of children ALL.The control group was from 17 cases normal health patients.Used real-time quantitative PCR method to detect the expression of lncRNA RP11-69I8.3 in the bone marrow of 64 cases of acute leukemia(32 cases of ALL and 32 cases of AML)and 25 cases of CR ALL and 17 cases of control group.Then analyzed the correlation index and significance between the result of expression of lncRNA RP11-69I8.3 and ALL patients' age,gender,initial white blood cell count,hemoglobin,platelet count,the type of T/B,percentage of bone marrow initial cells,the expression of BCR/ABL fusion gene,WT1 gene,chromosome karyotype and immunophenotype,extramedullary infiltration,whether remission after1 course of chemotherapy,whether relapse etc.Results1.Compared with the control group,there was no significant difference in the expression of lncRNA RP11-69I8.3 in AML group(P>0.05).The expression in the initial treatment ALL group was significantly lower than that in the normal control group(P=0.001).2.Compared with the initial treatment ALL group,the expression of lncRNA RP11-69I8.3 in CR ALL group after chemotherapy was significantly higher(P=0.013).3.In the primary treatment ALL,the expression of lncRNA RP11-69I8.3 in the children group was significantly lower than that in the adult group(P=0.017).There was no significant difference between the result of expression of lncRNA RP11-69I8.3 and ALL patients' gender,initial white blood cell count,hemoglobin,platelet count,T/ B of type,percentage of bone marrow initial cells,the expression of BCR/ABL fusion gene,WT1 gene,chromosome karyotype and immunophenotype,extramedullary infiltration,whether remission after 1 course of chemotherapy,whether relapse etc.4.In B-ALL,the expression of lncRNA RP11-69I8.3 is not related to the immunophenotype.Conclusion1.The expression of lncRNA RP11-69I8.3 in the initial AML was not significantly different from that in the control group.The expression of lncRNA RP11-69I8.3 was lower in the primary treatment ALL and higher in CR ALL group after chemotherapy.It suggested that the low expression of lncRNA RP11-69I8.3 may promote the development of ALL.2.In the primary treatment ALL group,the expression of lncRNA RP11-69I8.3in children was significantly lower than that in adult.There was no correlation between the expression of lncRNA RP11-69I8.3 and initial white blood cell count,percentage of bone marrow initial cells,the type of T/B,whether remission after 1course of chemotherapy,whether relapse et al.3.In B-ALL,the expression of lncRNA RP11-69I8.3 is not related to the immunophenotype.