| Objective: Acute myeloid leukemia(AML)is a malignant tumor of the hematopoietic system.With current standard treatment,approximately 35% to 40% of low-risk young AML patients can be cured.AML is a malignant blood disease with a high incidence in the elderly,with a median age of 65 to 70 years.However,due to its special clinical and biological characteristics,the remission rate of chemotherapy is only 45%,the 5-year survival rate is only about 10%,and the early mortality rate is as high as 30%.In recent years,although the research on leukemia has made some progress,it still faces severe challenges.Long non-coding RNAs(lncRNA)is an RNA transcript with a length of more than 200 bp nucleotides and a lack of protein-coding capacity.LncRNA plays an important role in various biological processes and the development of cancer.At present,more and more studies have shown that lncRNA may regulate gene expression at both transcriptional and post-transcriptional levels in epigenetics.LncRNA can be abnormally expressed in a variety of cancers and play an important role in promoting and maintaining the development of tumors,demonstrating the important clinical potential of lncRNA as a biomarker and therapeutic target.With the development of sequencing technology,leukemia-related genes are attracting increasing attention,among which lncRNA has become a hot topic in leukemia research.However,the current research on lncRNA as a molecular biomarker to predict the prognosis of elderly(over 60 years old)AML patients is still incomplete.The purpose of this study was to screen the information related to the prognostic effect of differentially expressed lncRNA and their downstream target molecules on AML patients aged over 60 years by analyzing the TCGA database.There is no doubt that it is of great significance for prognosis and treatment if the development mechanism of lncRNA in elderly and high-risk AML patients can be further studied and appropriate lncRNA can be found as a therapeutic target or as a marker of new individualized treatment.In this study,TCGA data were analyzed to screen differentially expressed lncRNA and its downstream target molecules,and the relevant information on the prognostic impact of AML patients over 60 years old was analyzed.In this study,TCGA data were analyzed to screen differentially expressed lnc RNA and its downstream target molecules,and the relevant information on the prognostic impact of AML patients over 60 years old was analyzed.Methods: AML patients over the age of 60 from the TCGA database were divided into two groups according to the NCCN guidelines: high risk AML and low to moderate risk AML.Differentially expressed lncRNA/miRNA/mRNA were screened by "edgeR" in R software.Survival analysis of lnc RNA differentially expressed in high-risk AML and low-to medium-risk AML was conducted using the "survival" package in R software,and ceRNA network was constructed.The expression of lncRNA in bone marrow specimens of patients with clinical patients was verified by RT-qPCR.Results: Based on lncRNA/mi RNA/mRNA expression data in TCGA database,17 out of 22 lncRNA(AC009154.1,AC011124.1,AC084819.1,AC087258.1,AC092691.1,AC093627.2,AC117402.1,AC144450.1,AL022396.1,AL035691.1,AL138899.1,AL355974.2,AL441943.2,LINC00703,LINC00871,LINC01360,LINC01612)were negatively correlated with prognosis.5 lncRNA(LINC00648,AC103702.2,AC100775.1,AC092484.1,AC091138.1)were positively correlated with prognosis.The ce RNA networks related to these LncRNA were obtained and the GO and KEGG pathways of mRNAs were analyzed.Finally,RT-qPCR was used to verify the bone marrow specimens of 32 patients aged over 60 years old with high risk and 16 patients with low to moderate risk AML in our hospital,in which AC093627.2 was differentially expressed(r = 0.167,0.918,P < 0.05).Conclusion: The expression of AC093627.2 in elderly patients with high-risk AML was significantly higher than that in low-risk patients.The differential expression will contribute to the judgment of prognosis and the choice of treatment. |
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