| Objective:This study is using Baijie Powder,an experienced prescription in our department,to help wounds that with Crohn's anal fistula dressing after surgery,and detailed records in the two groups after treatment in patients with surgical wound healing rate,overall curative effect evaluation,and watch the granulation tissue fibroblasts count after dressing,transforming growth factor beta(TGF-beta 1)and alpha smooth muscle actin(alpha SMA)expression and the achievement of the wound the change of the amino acid content,then evaluating the clinical effect of Baijie Powder on wound healing of crohn's disease after anal fistula.This study is a preliminary study on the mechanism of Baijie Powder promoting the wound healing of crohn's disease anal fistula,starting from the medical treatment and combining with the characteristics of the dispersant.The purpose is to shorten the postoperative treatment time,providing a new thought for reducing pain,and providing theoretical basis for the Baijie Powder's clinical widely use.Methods:In this subject,40 patients were admitted to the hospital of the first affiliated hospital of anhui university of Chinese medicine in November,2016-november,2017.Among them,27 males and 13 females were aged between 14 and 54 years old,and all patients were randomly divided into two groups,20 patients in the treatment group and20 in the control group.The two groups of patients began to change medicine on the second day after surgery,and once a day.The treatment group was used to fill the wound with vaseline gauze that contains 2g Baijie Powder/cm~2,and the control group filled the wound with vaseline gauze,and recorded the general information of the two groups,such as gender,age and original area of wound surface;The wound healing rate of the patients was 7d and 14d after surgery.Histopathological observation of the number of fibroblasts in granulation tissue;The expression of TGF-?1 and?-SMA were analyzed by Western blot.Enzyme linked immunosorbent assay(ELISA)was used to detectchanges in hydroxyproline content.The overall efficacy was observed on the 30th day after surgery;The whole research process observes and records the occurrence of adverse events.Statistical analysis was performed using statistical software(SPSS 21.0)and objective evaluation was carried out.Results:The gender,age and original area of the two groups were compared before the treatment,we find that the differences were not statistically significant and comparable(P>0.05).What was exciting was that both groups had no adverse events during the treatment.The results are as follows:(1)Comparison of activity index score of crohn's disease in the two groups:Comparison of CDAI between the two groups before and after operation on 14th day was statistically significant,with no statistically significant difference between the two groups,indicating that the surgery and the Baijie Powder had no significant effect on the condition of crohn's disease.(2)The wound healing rate and total efficacy evaluation:The two groups in postoperative wound healing rate evaluation on the 7th day,14th day was statistically significant(P<0.05),which shows that the treatment group was superior to the control group in promoting wound healing and improving wound healing rate.On the 30th day after surgery,the overall efficacy of the treatmentgroups was 100%,and the control group was 90%.We find the difference was statistically significant by using the rank sum test analysis(P<0.05),indicating that the overall efficacy of the treatment group was better than that of the control group.(3)The fibroblast count in the wound granulation tissue:The fibroblasts count in treatment group were higher than control group on 7th day and 14th day after surgery,and the difference has statistical significance(P<0.05).It is means that the treatment group is better than that of control group in terms of promote wound healing.(4)The expression of TGF-?1 and?-SMA:Compared with the control group,the expression of TGF-?1 and?-SMA was significantly higher in the treatment group than in the control group on 7th day and 14th day after surgery(P<0.05),indicating that the treatment group was better than the control group in regulating the expression of TGF-?1 and?-SMA.(5)The hydroxyproline content:The content of hydroxyproline in the treatment group was higher than that in the control group on 7th day and 14th day after surgery(P<0.05),indicating that the treatment group was superior to the control group in the increase of hydroxyproline acid content.Conclusion:This topic research has shown that the Baijie powder can improve wound healing rate,increase the granulation tissue fibroblasts count and regulate the expression ofTGF-?1and?-SMA,alsocanincreasehydroxyprolinecontentof wound.Therefore,Baijie powde can promote wound healing and improve clinical efficacy,of course it's safe.Now,we can say it can be widely used in the treatment of crohn's anal fistula. |
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