Effect And Mechanism Research Of Ethanolic Extracts Of Lamiophlomis Rotate On Proliferation Of MEC-1 Cells

Objectives: This research is conducted to reveal the inhibitory and apoptotic effect of EELR upon MEC-1 cells and to primarily discuss the relevant molecular mechanism.Methods: To determine the cell vitality with SRB colorimetry assay;to examine the cell proliferation rate through the cloning formation assay;to test the cycle arrest and the subsequent apoptosis rate of MEC-1 cells by means of flow cytometry(FCM)and Hoechst33258/PI fluorescence staining;to use Western Blot for the assay of the apoptosis-related protein expression.Results:1.EELR at different concentrations inhibited the proliferation of MEC-1 cells in a time-and-dose-dependent manner;besides,EELR exhibited obvious inhibitory effects on the proliferation of MEC-1 cells within continuous 7d.2.EELR at different concentrations took effect on MEC-1 cells after the forty-eight-hour examination through flow cytometry(FCM): the apoptosis rate of MEC-1 cells displayed a marked difference,and the cell cycle exerted visible changes with the cells being arrested in G2/M phase.3.Hoechst/PI staining showed that,with the increase of EELR concentration,the total number of cells within the same view demonstrated a negative correlation,while the proportion of apoptotic cells was positively correlated with necrotic cells.4.When the EELR concentration is less than or equal to 100 g/mL,it exerted no inhibitory effect on Chang-liver cells;in addition,no remarkable difference was observed when compared with the normal control group(P>0.05).5.EELR at different concentrations showed great inhibitory effect on the clone formation capacity of MEC-1 cells(P<0.01).6.The result of Western Blot examination showed that the Bcl-2 protein expression of MEC-1 cells would reduce while Bax(P<0.01)and Casepase-3 protein expressions elevate(P<0.01)as the EELR concentration increased.Conclusion:1.EELR can inhibit the proliferation,induction and apoptosis of MEC-1 cells cultured in vitro without marked cell toxicity;2.EELR can induce cells to apoptosis by means of regulating the expression content of Bcl-2,Bax and Casepase-3 in MEC-1 cells.