The Pathogen Identification Of Widespread Dermatomycosis And Analyse Of Pathogenic Factors In Patients

Objectives:To investigate the distribution of pathogenic dermatophyte species and make clinical analysis in patients with widespread dermatomycosis,preliminarily survey if there is the difference between the distribution of pathogenic dermatophyte species in patients with widespread dermatomycosis and tinea corporis or tinea cruris.To explore the pathogenic factors in patients with widespread dermatomycosis.Methods:1.We collected clinical data of widespread dermatomycosis(experimental group)and tinea corporis or tinea cruris(control group)in the Affiliated Hospital of North Sichuan Medical College from Jan.2016 to Dec.2016.The dermatophytes isolated from patients were observed by microscope and cultivated in SDA medium,the DNAs of dermatophytes were amplified by the primer Its1 and Its4.Dermatophyte species were identified by blasting the PCR produces in GenBank.Finally,we analysed the difference of the distribution of pathogenic dermatophyte species between experimental group and control group.2.We collected patients' clinical data of experimental group and control group to analyse the pathogenic factors of the widespread dermatomycosis.Results:1.Among the experimental group,the patients are from 3 to 85 years old.Their average age is 39.86±3.37 years.The average course time is584.92 ± 132.52 days and the proportion of male and female is 1?0.54.51.4% of the patients are over 40 years old.The proportion of city and village is 1?1.18.Among the control group,the patients are from 2 to 70 years old.The average age is 31.20±3.01 years.The average course time is 67.37±14.54 days and the proportion of male and female is 1?1.19.31.4% of the patients are over 40 years old.The proportion of city and village is 1?0.75.2.All the lesions in every patient shared the same pathogen in experimental group.Four species of dermatophyte were identified,T.rubrum(25samples),T.mentagrophytes(9samples),M.gypseum(2samples),T.tonsurans(1sample).As for control group,three species of dermatophyte were identified,T.rubrum(27samples),T.mentagrophytes(7samples),M.gypseum(1sample).There was no distinction in the pathogenic dermatophyte species of two groups by SPSS statistical software.3.From the clinical data,we found that32 patients have used glucocorticoid(topical 29,oral 1,topical and oral 1,topical and inhale 1)for a long time,8 patients have touched animals,7patients have diabetes,3 patients have hypertension,2 patients have SLE,1patient has asthma and 1 patient has tumour in the experimental group.In the control group,5 patients have used glucocorticoid for a short,5 patients have touched animals,3 patients have diabetes.Conclusions:1.All the lesions in different parts of one patient share the same pathogen.2.There is no distinction in the pathogenic dermatophyte species of two groups.It is not the specific dermatophyte species to cause widespread dermatomycosis.3.The patients of two groups are not the same in age,course time,using glucocorticoid,underlying disease and so on.These factors above may be the related factors to cause widespread dermatomycosis.