| Research purposeAlcohol is one of the important factors that cause liver injury,the modern society with the change of the people's living habits,alcoholic liver damage(ALD)injury incidence rate has a rising trend year by year,and the related research mainly focused on the pathogenesis,progress and the research on the method of prevention and cure of ALD is slow,although the clinical drugs for prevention and cure of ALD is more,but the effect is not very ideal,or there are many disadvantages,so the research a kind of prevention and cure of ALD is safe and effective new drugs is necessary.Leaves off gentian(Gentianella acuta)as the gentianaceae false gentian belong to a kind of,also gentian.Advocate in the greater hinggan mountains north,west,east Mongolia plateau,mountain,helan mountain,as a local medicine to whole herb medicine,attending icteric model hepatitis,cholecystitis.Modern research proves,pointed leaves off gentian has significant antioxidant activity and the effect on the regulation of certain metabolic enzymes,ALD onset process is mainly composed of oxidative stress,the activation of certain genes involved in,so it is possible that leaves false gentian has the function of prevention and cure of ALD.This experiment mainly through the establishment of the rat model with chronic ALD,determination of liver damage enzyme indexes and observation of liver pathological changes to verify pointed leaf gentian protect liver function;Determination of liver enzyme system of ethanol metabolism,antioxidant system,indices such as gene expression studies pointed leaves protect liver mechanism of gentian.Research methodsHealthy male SD rats were randomly divided into six groups,gradient alcohol lavage or amount of normal saline for eight weeks,to establish the rat model of chronic ALD,since 7 weeks been given daily doses of leaves off gentian extract or east treasure for liver lavage,determination of rat liver volume ratio on the 8th weekend,activity of serum AST,ALT,content of MDA,TG,GSH content and SOD activity in liver homogenate and liver pathology change,to evaluate leaves off gentiana the effect of prevention and cure of ALD.Determination of FFA in serum of rats,TNF-a,NEFA content,ALD,HADH content in liver tissue homogenate,5-HT content in hippocampus,CYP2E1 expression in liver cells level to explore possible mechanisms of leaves false gentian antagonism ALD.The results of the study1.Leaves off gentian effects on liver damage indexIn rats was made with 50%ethanol after liver cell and tissue damage is serious,abnormal liver tissue and biochemical indicators.Leaves reaction significantly reduce false gentian each dose group pathological changes,liver tissue and serum biochemical index levels close to normal.Application pointed leaves after false gentian,MDA in serum of rats,AST,ALT,TG concentration is reduced,the concentration of GSH in liver homogenate is lower;Increase the concentration of SOD in the liver homogenate.Have prevention and cure function on chronic alcoholic liver damage.2.Leaves off gentian of the body's antioxidant system,metabolic enzyme system,gene expression and neurotransmitter factors such as the influence of some indicatorsRats after being filled with 50%ethanol,pointed/false gentian each dose group of FFA in serum of rats,reduce the content of TNF alpha,adiponectin levels rise;ALDH,ADH content increased in liver tissue,5-HT content in brain hippocampus;In hepatocyte CYP2E1 expression level decreased obviously.conclusion1.Leaves off gentian for the prevention and treatment of chronic ALD has obvious effect.2.Leaves off gentian antagonism chronic ALD possible mechanisms are:to promote the production of adiponectin,increase the consumption of fat,reduce liver damage;Promote ALDH,ADH secretion,improve the metabolism of alcohol;Regulate the expression of CYP2E1,inhibit excessive expression and reduce its activity to reduce oxidative damage of liver;Inhibiting the production of TNF alpha,FFA,to reduce the inflammation of the liver. |
PDF Full Text Request