Study Of The Anti-temulence Effect And Protective Mechanism Of Liver-protective Oral Solution On Acute And Chronic Alcoholic Liver Injury

In order to evaluate the anti-temulence effect of Liver-protective Oral Solution and its protective mechanism on acute and chronic alcoholic liver injury.In this study,the drunk model was used to determine the best dose;drunken sleep experiments were used to compare the the anti-temulence characteristics of Liver-protective Oral Solution;by measuring the change of ethanol content in temulence mice,the effects of Liver-protective Oral Solution on alcohol metabolism were studied;by establishing an acute and chronic alcoholic liver injury model,the activity of AST,ALT and SOD,the level of GSH,MDA were measured;HE staining sections were used to observe the changes of liver morphology.The study found that the best dose of mice induced dose was 11ml/kg.Compared with the model group,it can prolong the ebriety time and shorten the sober-up time(p<0.05);the acute and chronic alcoholic liver injury model of each dose group can significantly reduce the activity of the serum AST,ALT(p<0.05);high and medium dose group can reduce blood ethanol content at 2 h,3 h(p<0.05).In the model of acute alcoholic liver injury,the levels of SOD and GSH increased(p<0.05)and the levels of MDA decreased(p<0.05)in the liver of each group,while in the model of chronic alcoholic liver injury,the levels of SOD and GSH had no significant difference in the levels of SOD,GSH and MDA in the dose group.HE staining sections showed that liver injury caused by ethanol in the liver tissue was significantly improved in the acute,chronic alcoholic liver injury model.High and medium dose group is better.In this study,we preliminarily confirm that Liver-protective Oral Solution can significantly prolong the drunk time,shorten the sober-up time,reduce the blood ethanol content and have a better protective effect in alcohol-induced liver injury.