| Purpose:Experimental use of alcohol toxicity method to copy the rats with alcoholic liverinjury in animal models, modeling and administration at the same time, thegeneral condition of the rats with alcoholic liver injury in animal models byobserving Yigan particles, liver index, serum ALT, AST, GGT, TNF-alpha, measuredin the liver homogenate MDA content and SOD activity and tissue morphology andwith Yinzhihuang particles compared to clarify liver and mechanism of actionof the particles prevention and treatment of alcoholic liver injury in rats.Method:1. The group made mould and intervention therapyAdaptive fed the rats for3days to adapt to the laboratory environment, collectedcaudal vein blood, the serum levels of ALT, AST were determined to exclude theimpact of non-health factors. According to the weight which meets the conditions,rats were randomly divided into five groups: the normal control group, the modelgroup, the low dose Yigan particles group, the high dose Yigan particles group,the Yinzhihuang particles group, the quantities of the male was equal to thefemale. The normal control group used equal amounts distilled water stomach tube.The model group were given wine7ml/Kg per time, twice a day, time interval offour hours, after one hour the wine were gave, gave equal amounts distilled waterstomach tube. The low dose Yigan particles group gave Yigan particles watersolution0.39g/ml,7ml/kg per time, once a day after one hour the wine were gave,the rest of the disposal was consistent with the previous group. The high doseYigan particles group only changed the Yigan particles water solution to0.78g/ml,the rest of the disposal was consistent with the previous group. The Yinzhihuangparticles group after one hour the wine were gave, gave0.012g/ml Yinzhihuangparticles water solution,7ml/kg per time, once a day. The experiment lasted28days, drawn the materials in the29th day.2. Observation of the general status During the experiment, mental status, diet, toilet case, reaction after gavethe wine, the change in color, weight, deaths and so on were observed in rats.3. The experimental indicators drawn and detectionEach group drawn the materials in the29th day, before the day, each experimentalgroup rats were fed nothing but water for12hours. Weighing in the drawn day,gave10%Chloral hydrate solution by intraperitoneal injections, after the ratswere anesthetized (about20minutes), Cut the rat’s abdominal cavity, lookedfor the abdominal aorta quickly and took blood2ml×2canal, put the rats todeath, removed the liver rapidly, weighed and calculated liver index. Serum thelevels of ALT,AST,GGT,TNF-α.Took the left lobe of the liver tissue homogenate,measured the MDA content and SOD activity, took the right lobe of the liver tissueabout100mg into10%formaldehyde solution, did optical microscope observation.Result:1. Experimental rat model of alcoholic liver injury alcohol toxicity test methodcan be successfully.2. Yigan particles can reduce alcoholic liver injury model rat serum AST, ALT,GGT level3. Yigan particles can reduce alcoholic liver injury in a rat model of MDA content,SOD activity, and better than Yinzhihuang particles.4. Yigan particles can reduce the impact of TNF-α levels in alcoholic liverinjury model.5. Yigan particles from the pathological point analysis can reduce hepaticsteatosis, reduce liver cell necrosis, reducing inflammatory infiltration ofthe liver cells better than Yinzhihuang particles.Conclusion:Yigan particles by protecting the liver cells, and reduce the permeability ofcell membranes to reduce the AST the release, to reduce the generation of ALT,GGT. Maintain the liver promote oxidation system and antioxidant systems existhomeostasis, and reduce the occurrence of lipid peroxidation, reduce malondialdehyde content, reduce the level of oxygen free radicals liver.Increased SOD activity in the liver to improve its content can acceleratescavenging oxygen free radicals. Yigan particles can reduce the generation ofendotoxin, blocking the formation of TNF-α, mitigate aroused a series ofinflammatory reactions due to the TNF-α release. Reducing steatosis to reduceliver cell necrosis, reducing inflammatory infiltration of liver cells, playthe purpose of prevention and treatment of alcoholic liver injury. And betterthan Yinzhihuang particle group. |
