The Risk Correlation Study Of Genetic And Environmental Factors And Benign Prostatic Hyperplasia And Prostate Cancer

Part one The risk correlation study of genetic and environmental factors and benign prostatic hyperplasiaObjection To investigate the environmental factors and the genetic characteristics of the variability of the seven genes associated with Benign prostatic hyperplasia.Methods This was a case-only study,By literature search and analysis of bioinformatics research,7 SNPs in 7 genes,which were IGF1(rs5742714)?ACPP(rs1126661)?GSTA1(rs4715333)?CHRM2(rs8191993)?PDE5A(rs113890665)?TFF1(rs2156310)and MKI67(rs8473)were selected.we divided the 409 BPH patients into two groups by the prostate volume ? 30 ml or <30ml.Genotyping the 7 SNPs by sequenom technology,analyzing and comparing the environment factors and the genotype and allele frequency distribution between different groups.Results The culture degree(p=0.001),work types(p=0.007),whether to have the basic medical insurance(p=0.006,OR=2.03,95%CI=1.22-3.41),urgency(p=0.006),and texture of the prostate(p=0.002)were significantly associated with prostate volume of BPH patients.Of all the 22 indirect risk factors,The PSA concentration was significantly different between the group of prostate volume less than 30 ml and greater than OR equal to 30 ml(p <0.001),prostate volume acuity 30 ml PSA concentration significantly higher than the prostate volume < 30 ml of BPH patients.Based on the analysis of 7 different genotype SNPs,We found that only one gene(PDE5A gene rs113890665 loci(A/G))was associated with patients' different prostate volume with BPH(p = 0.048).the risk allele A will increase the risk of BPH patients with prostate volume larger.Conclusions 1 The education level,occupation and many other environmental factors play an important role on the occurrence and development of benign prostatic hyperplasia.2 rs113890665 PDE5 A gene(A/G)locus mutation may promote the occurrence and development of benign prostatic hyperplasia.Part two The correlation study of genetic factors and prostate cancerObjection To explore the genetic characteristics of the variability of the eight genes associated with prostate cancer.Methods This is a case-control study.For the 8 SNPs of ABCA1 gene,we genotyping221 PCA cases and 477 normal controls by HRM technology.We use haploview 4.0 and SPSS 17.0 software analysis,explore the ABCA1 gene and genetic correlation of prostate cancer.Then use sequenom technology genotyping of 373 patients with PCA and 721 normal controls,aimed at seven sites of the first part of study.We performed the genetic correlated with prostate cancer case-control study,explore the seven sites of different genotypes and alleles and the risk of prostate cancer.Results 1 7 genes in the study of the second part,in view of the particular SNPs loci results of case-control study,ACPP gene rs1126661 loci different genotype distribution between the two groups had significant difference(p = 0.041),the alleles have significant difference in the distribution of cases in the control group(p = 0.045,OR = 0.714,95% CI =0.513-0.994),the dominant model has significant difference between the two groups(p =0.035,OR = 0.68,95% CI = 0.48-0.97),the ultra-dominant genetic model also have significant differences between the two groups(p = 0.033,OR = 0.68,95% CI = 0.48-0.97),Co-dominant model analysis,the CC/CT genotype distribution between the two groups have significant difference(p = 0.033,OR = 0.68,95% CI = 0.482-0.972),the additive genetic model distribution between the two groups have significant difference(p = 0.042,OR = 1.42,95% CI = 1.013-1.991).rs8473 MKI67 gene loci dominant model has a significant difference between the two groups(p = 0.041,OR = 1.32,95% CI = 1.01-1.74),Co-dominant modelanalysis,the TT/CT genotype distribution also has a statistically difference between the two groups(p = 0.030,OR = 0.729,95% CI = 0.548-0.970).2 in view of the eight SNPs of ABCA1 gene loci specific case-control studies,as a result,only rs2275542 associated with PCA risk(p = 0.018),and other sites in the PCA cases and control group had no significant difference,but the haploid type analysis,found the multiple protective haploid type and risk haploid type,such as CAGTTGCC(p = 0.011,OR =0.011,95% CI = 0.119 0.805),CAGTTGTT(p = 0.001,OR = 0.001,95% CI = 0.014 0.549)and CGGTCACT(p = 0.025,OR = 0.025,95% CI = 0.302 0.931)are protective factors,type and haploid CAGTTGCT(p = 0.001,OR = 0.001,95% CI = 2.402 10.938),CGGTCACC(p= 0.001,OR = 0.001,95% CI = 1.654 5.350),TAACCGTC(p = 0.001,OR = 0.001,95% CI =1.856 11.955)and TGACCACC(p = 0.007,OR = 0.007,95% CI = 1.141 2.389)is a risk factor.Conclusions 1 Only ACPP gene rs1126661 loci in the 7 genes exist significant correlation with prostate cancer.2 Only rs2275542 of ABCA1 gene is associated with PCA risk.3 The haploid type analysis found that there were multiple protective haploid types and risk haploid types.