Research And Application Of Biocompatible And Environmental Responsive Hydrogels

Biomaterials are interdisciplinary fields.The research contents include materials science,life science,chemistry,biology,physics and so on.In recent years,the area of biomaterials is hot and is also considered as having broad prospects for development.Hydrogel is a three-dimensional hydrophilic polymer network,which can be made from a variety of natural and synthetic polymers.It can be designed and fabricated into high water-contain materials or tissues such as articular cartilage,so it is considered as an ideal biomaterial.With the development of the field and the need of practical application,more and more researchers are focusing on the design of hydrogels with biocompatibility and environmental responsiveness,namely "smart hydrogel",to find a better way to apply hydrogels to biomedicine and other fields.In the first chapter,we mainly introduced backgrounds,the materials and methods used in the researches.Firstly,we give the hydrogel a brief introduction.Secondly,the preparation methods and ideas of hydrogel are classified,and the current demand situation for biocompatible biomaterials is summarized.Then two related topics are introduced.First,the introduction of the environmental responsive hydrogel and the Diacerein which is mainly used in the first part of experiments is mentioned.Secondly,a detailed description of the bacterial plasmid partioning system ParRMC,which is involved in the second topic,and a brief introduction of the tensegrity structure model used in the second experiment is given.In the second chapter,we focus on the topic of environmental responsive hydrogel we made which can be used as a drug release system.We synthesized the supramolecular hydrogel with PEG,graphene oxide and diacerein through chemical synthesis and physical mixing,which has good biocompatibility.We studied the mechanical properties of the hydrogel by rheological method and found that the hydrogel was injectable.In addition,the drug release kinetics of drug loaded hydrogels under ultrasound stress showed that the hydrogel had controllable drug release effect.Further experiments using the hydrogel to culture the cells showed that.it had the ability of tissue repair,and verified its biocompatibility and environmental response ability.In the third chapter,the idea of making hydrogel by combining the ParRMC system with the tensegrity structure is discussed.The ParRMC system,meaning bacterial plasmid partioning system,has a specific response to ATP and can form complex protein assembly structures.We try to combine this property of the ParRMC system with PEG to produce a special hydrogel with a tensegrity-like structure,which is also responsive to ATP.We have carried out a series of experiments to verify the properties of the ParRMC system,and discussed and tried the method of making hydrogel.Finally,we make a summary of all the work we talked above,discuss the results obtained at present and look forward to the follow-up work needed.