Clinical And Experimental Research On Painful Diarrhea Fomula's Treatment Of Postoperative Diarrhea Of Colon Cancer

Objective:Through clinical contrast,observe the curative effect that painful diarrhea fomula has on postoperative diarrhea of colon cancer;and from the perspective of aquaporin,observe whether Chinese medicine has promoted the expression of aquaporin3(AQP3)and aquaporin8(AQP8)in colonic mucosa,has strengthened the colon absorption of moisture and has shortened the compensatory period of postoperative diarrhea of colon cancer,to achieve therapeutic effect and provide certain theoretical support for traditional Chinese medicine treatment of postoperative diarrhea of colon cancer.Methods:(1): In the clinical research: 40 patients who conform to the diagnostic criteria of colon cancer postoperative diarrhea were randomly divided into control group and treatment group,20 cases in each group.The treatment group members take painful diarrhea fomula decoctum;the control group members take loperamide hydrochlo-ride capsules.4 weeks later,analyze and compare the general curative effect?improvement of major symptoms?accompanying symptoms and individual symptoms by syndrome integral.(2)In the animal experiment: 36 SD adult male mice were randomly divided into blank group(group A),the operation model group(group B),painful diarrhea fomula group(group C)and loperamide group(group D),9 mice in each group.Except group A,other three groups are treated with Subtotal colectomy after general anesthesia and in the first three days,are treated by intraperitoneal injection of gentamicin line.The group C and group D separately take painful diarrhea fomula and loperamide lavage for a week from the first day after surgery.On the 7th day,14 th day and 28 th day,kill 3 rats of each group.Anastomotic colon were selected to observe the expression of aquaporin3 and aquaporin8 after immunohistochemistry.Results:(1)In the clinical research: the general curative effect ?the curative effect of main symptom and secondary symptoms are better than control group(P < 0.05),the curative effect of thin stool? the increasing of stool times ?abdominal pain and abdominal distension of the treatment group are better than control group(P < 0.05),the curative effect of bowel flatus ? the pain of stomach duct and abdomen ?inappetence?thirsty and belching of the treatment group are same with control group(P >0.05);(2)In the animal experimentation: the expression of aquaporin3 and aquaporin8 in B,C,D groups was higher than group A on 7?14?28 days after surgery(P < 0.01);the expression of aquaporin3 and aquaporin8 in C,D groups was higher than group B on 7?14?28 days after surgery(P < 0.01);the expression of aquaporin3 and aquaporin8 in C groups was higher than group D on 7?14?28 days after surgery(P < 0.05).Conclusion: Painful diarrhea fomula has curative effect on postoperative diarrhea of colon cancer,which is related to that Tongxieyaofang promote the expression of aquaporin3 and aquaporin8 in the colonic mucosa after operation.