Meaning Of Glucose-6-phosphate Isomerase In Rheumatoid Arthritis

Objective:To determine the diagnostic value of glucose-6-phosphate isomerase(GPI)in rheumatoid arthritis(RA),and to explore its relationship with disease activity.Methods:A total of 300 patients with RA in our hospital were recruited from December 2015to December 2017,and the control group was 100 patients with other autoimmune diseases.Gender and age were recorded.GPI was detected by enzyme-linked immune sorbent assay(ELISA).Erythrocyte sedimentation rate(ESR),c-reactive protein(CRP),rheumatoid factors(RF),anti-cyclic citrullinated peptide(CCP)antibody,anti-keratin antibody(AKA),mutated citrullinated vimentin(MCV),anti-perinuclear factor(APF)were detected as well.To investigate the diagnostic value of GPI in RA.Ninety-one RA patients with completed data were collected,including height,weight,tender,swollen and GPI,ESR,CRP,RF.Body mass index was calculated.The disease activity score 28(DAS28)was evaluated at the same time.Among which 31patients followed up were collected,before and after treatment.Concentration of GPI,tender,swollen,ESR,CRP,RF were compared.To explore the relationship between GPI and disease activity.Data was analyzed by SPSS 21.0.Materials were indicated with`x±s.Comparison between groups was performed withχ2 test or t test.Pearson liner regression was used for correlation analysis.The sensitivity and specificity of GPI and related autoantibodies to RA were calculated.P<0.05 indicates the difference significantly.Results:1.The positive rate of GPI in RA was 26.3%(79/300),and 2%(2/100)in control group(χ2=20.5,P<0.001).2.The ESR,CRP and RF of the 300 RA patients with positive GPI were significantly higher than those of the negative patients(t=3.833,P<0.001;t=6.032,P<0.001).3.The sensitivity of GPI to RA in 300 RA patients was 26.3%,the specificity was98%,and the positive predictive value was 97.5%.The sensitivity was increased evidently when tested together with anti-CCP antibody,AKA,MCV or APF compared with GPI and other related autoantibodies respectively(χ2=21.66,P<0.001;χ~2=12.46,P<0.001;χ~2=15.80,P<0.001;χ~2=35.97,P<0.001).4.In 300 RA patients,the detection rate of GPI was 7.5%(4/53),14.6%(18/122),3.5%(2/57)and 15.3%(19/124)respectively when anti-CCP antibody,AKA,MCV,APF were negative,with the value of supplementary diagnosis.5.GPI was positively correlated with tender,swollen,ESR and DAS28 in 91 RA patients with completed data(r=0.347,P=0.001;r=0.330,P=0.001;r=0.210,P=0.045;r=0.350,P=0.001).6.GPI,tender,swollen,ESR and DAS28 decreased after treatment in 31 RA patients(t=2.255,P=0.029;t=5.116,P<0.001;t=3.616,P=0.001;t=3.053,P=0.003;t=7.115,P<0.001).Conclusion:1.GPI has auxiliary diagnostic value for RA.2.The GPI is positively correlated with the activity of RA,and may be used as the indicator of disease monitoring and guidance for treatment.