The Diagnosis Significant Gluocose-6-phosphate Isomerase In Patient With Rheumatoid Arthritis

Objective: To investigation the diagnosis significance of gluocose-6-phosphate isomerase(G6PI)detected by ELISA in patients with rheumatoidarthritis(RA). (2)To compare the correlation of the G6PI and anti-CCP andIgM rheumatoid factor(RF).(3)To analysis the relation of G6PI withpathogentic condition activity index.(4)To contrast the relation of 6GPIand anti-cyclic citrullinated peptide antibody (anti-CCP),RF,Antikeratinantibodies(AKA) with anticular erosion。Material and Methods: The G6PI was detected by an enzyme linkedimmunosorbent assay (ELISA) in serum sample from 106 patient withRA,53 non-RA control with various rheumatic disease, and healthyIndividual.The level of RF, anti-CCP antibodies and were alse assay in RApatients.The correlatin analysis a beween G6PI and anti-CCP, IgM-RF(rheumatoid factor). G6PI, anti-CCP,IgM-RF and AKA were conmaparebetween patient with erosion and with non-erosion disease.Reluts: (1)G6PI serum level in patients with RA were 1.611±1.196ug/ml,and were 0.106±0.174 in other rheumatoid disease ,and 0.056±0.086ug/ml in healthy individual.There are significant statistic differences betweenpatient and other rheumatoid disease. (P＜0.05) Receiver operator curveanalysis(ROC) showed an opitium cut off level for G6PI at 0.225ul/ml.Thesensitivity of G6PI was 0.868,the specificity was 0.853 in RA.G6PI is association with RF, but not not association with anti-CCP.G6PI were notassociated with disease activity index by spearman correlation analysis.Theassociation between above parameters with bone erosion was notdetected,however.Conclusion:The G6PI that abnormally increased in some RA may beto a new disgnostic marker in RA.G6PI presents a reasonable sensitivity with(86.8％)with high specificity(85.3％) to RA and it is valuable to RAdiagnosis.G6PI is association with RF, but not completely overlaps them.G6PIis not associated with diseases activity index. No associtionnn is foundbetween G6PI and bone erosion.