| Objectives:To explore the serum glucose-6-phosphateisomerase's (GPI) diagnosis value in rheumatoid arthritis (RA) patients;and to investigate the mRNA level of GPI expression in patients with RA and the relationship between GPI mRNA and RA in it's pathogenesis and activity.Methods:1.The serum levels of GPI were measured with ELISA, and also measured rheumatoid factor (RF-IgM, RF-IgG, RF-IgA)and anti-cyclic citrullinated peptide (CCP) antibody. The serum levels of RA patients of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR),immunoglobulin(IgG,IgA,IgM),complement(C3,C4) and platelet(Plt).2. The levels of GPI mRNA expression in the peripheral blood mononuclear cells (PBMCs) were detected in RA patients, the other autoimmune disease(AID) group and the health controls.Results:1.ELISA:RA group's GPI concentration was significantly higher as compared AID group and the health controls.There was no obvious difference between the early RA and the non-early RA in GPI level (P=0.43),and the same result happened between the active phase and stable phase (P=0.11).At a GPI concentration of 0.23mg/L, the sensitivity of test reached 67.2% and the specificity 68.0%.GPI was shown to be significantly related to the joint numbers with pain and rheumatoid factor (RF-IgM, RF-IgA) (P<0.05).There were no obvious difference between GPI,anti CCP antibody, RF-IgM and RF-IgA in terms of sensitivity for diagnosis of RA (P>0.05),but there was significant difference between GPI and RF-IgG (P<0.01). There were no obvious difference between GPI, anti CCP antibody, RF-IgG and RF-IgA in terms of specificity (P<0.01),but there was significant difference between GPI and RF-IgM(P>0.05).2.TR-PCR:The expression of GPI mRNA in RA patients was higher than AID group and the health controls (P<0.05).There was no obvious difference between the early RA and the non-early RA in GPI level, and the same result happened between the active phase and stable phase.Conclusion:1.GPI is a valuable diagnostic tool and a useful additional marker for RA. Combining GPI, anti CCP antibody and RF-IgA tests seemed useful to confirm RA patient who are RF negative.2.The increased expression of GPI mRNA may associate with RA, and it may play a pathological role in the pathogenesis of RA.3.The result of RT-PCR had a little different with ELISA. It maybe because of a fraction of GPI constructing GPI-anti-GPI and a fraction of GPI existing in inactivation. |
