Effect Of Tartary Buckwheat Resistant Starch On Intestinal Flora In Mice With Lipid Metabolism Disorder

With the improvement of people’s living standard,great changes have taken place in dietary structure,the proportion of dietary fat,and oxidative stress may induce lipid metabolism disorders,affect the balance of intestinal flora,endanger human body health.Buckwheat is rich in many kinds of functional factors,such as: buckwheat protein,flavonoids,resistant starch,and tartary buckwheat resistant starch are similar with dietary fiber physiological on the function,they can regulate blood sugar,blood lipid and treat constipation and diabetes.But at present,the research on resistant starch of Tartary buckwheat is mainly focused on its processing characteristics,and few studies have involved the physiological functions of Tartary buckwheat resistant starch.This topic with buckwheat grains as raw materials,first of all the pressure heat-enzyme hydrolysis and determination of resistant starch and structural characterization of the physical and chemical indexes,and then in preparation of buckwheat resistant starch as a raw material,to establish and optimize the hyperlipidemia animal model,based on the experimental animal physiological and biochemical,oxidative stress,inflammatory factors and the change of intestinal flora index,evaluation of the physiological functions of buckwheat resistant starch.Animal experiment: male C57 BL / 6 mice were randomly divided into 5 groups(each group 9): low fat feed group(LFD),high fat model group(HF-CS),buckwheat resistant starch intervention group(HF-BRS),corn resistant starch group(HF-CRS),buckwheat starch(HF-BWS).12 weeks after feeding,the analysis of the plasma and tissue of mice serum lipid metabolism related biochemical index,antioxidant activity index,and through the GC-MS and high-throughput sequencing technology analysis of main bacteria in the intestine in mice and its metabolite changes.The results are as follows:(1)The total starch content was 91.5%(w/w)and the content of resistant starch was 25.89%(w/w)in the prepared Tartary buckwheat resistant starch(BRS).The total starch content of corn resistant starch(CRS)was 90.6%(w/w),and the content of resistant starch was 20.39%(w/w).(2)The peak type of resistant starch is completely different from that of starch.However,the resistant starch peak type was relatively smooth,which was amorphous peak and resistant starch granules were vitreous.Buckwheat starch and corn starch peak type is similar,but the buckwheat starch had weaker peak in 20.456°2θ;The resistant starch of Tartary buckwheat was significantly different from that of maize resistant starch.The resistant starch of Tartary buckwheat had only a strong peak,while corn starch had four strong peaks.(3)BRS and CRS mice were significantly reducing blood fat index(TC,TG,LDL-C)and atherosclerosis index(AI)and lipid peroxide(MDA),raising HDL-C and organization of antioxidant activity(T AOC,SOD,CAT).At the same time,HF-BRS and HF-CRS in mice plasma inflammatory cytokines lipopolysaccharide(LPS),interleukin 6(IL-6),tumor necrosis factor alpha(TNF alpha)has dropped significantly in the HF-CS group(P < 0.05),suggesting that the BRS and CRS for caused by high fat diet inflammation has certain inhibition,which BRS inhibitory effect is superior to the CRS.(4)Microbial genome of mouse fecal samples was studied by high throughput sequencing technique.Analysis of the diversity of Alpha showed that the group of HF-BRS in bacterial diversity was higher than HF-CS.Analysis of Beta diversity(PCoA analysis)results showed that the significant gap between the five groups of mice feces samples,and BRS in the intervention group and the LFD group the difference between groups was less than the difference between the other three group and LFD group,showed that the BRS could adjust intestinal flora disturbance caused by high fat diet.