Preventive Effect Of Tartary Buckwheat Protein On The Dyslipidemia And Intestinal Flora In Mouse

With the improvement of people's living standard, great changes have taken place in dietary structure, the proportion of dietary fat, and oxidative stress may induce lipid metabolism disorders, affect the balance of intestinal flora, endanger human body health. Buckwheat is rich in many kinds of functional factors, such as:buckwheat protein, flavonoids, resistant starch, and buckwheat protein is a kind of difficult to digest protein, it has the function of the regulate lipid metabolism, but so far few studies involving buckwheat protein effect on intestinal flora and its associated with regulating blood lipid metabolism. Therefore, this article through dietary lipid metabolic disorder induced mouse model to explore the buckwheat protein on intervention high lipid metabolic disorders, oxidative stress and the link between the role of intestinal flora imbalance.(1) High fat diet can cause blood lipid metabolic disorder and insulin sensitivity in mice (ISI) decreased significantly (P< 0.05), oxidative stress, PBF and BWP can significantly reduce the blood lipid ((TC, TQ LDL-C), glucose (GLU, INS) and atherosclerosis index (AI) and lipid peroxide (MDA) content, raise the content of HDL-C, ISI and the antioxidant activity of organize (T-AOC, SOD, CAT), and basically recovered to the level of the blank group.(2) Compared with PBF and HFD, the apparent digestibility of protein and crude fat significantly reduced by 9.76% and 4.06% respectively, BWP group significantly decreased 6.93% and 2.05%. As containing PBF and BWP feed intake, a significant increase in the total bile acid and cholesterol in the feces excretion in mice, the correlation analysis showed that the total bile acid and cholesterol excretion and HDL-C and ISI was significantly positively related. At the same time, PBF and BWP acetic acid, propionic acid, butyric acid content were significantly higher in the HFD. Suggesting that buckwheat protein difficult to digest and undigested protein adsorption bile acid and cholesterol in vitro, may thus intervention lipid metabolic disorder.(3) Through the plate count and PCR technology for the type of main bacteria in the intestine (bifidobacterium, lactobacillus, escherichia coli, enterococcus) for testing, the results almost the same change trend, namely the group of PBF and BWP's beneficial bacteria number (bifidobacterium, lactobacillus, enterococcus) were significantly higher than that of HFD, harmful bacteria (escherichia coli) are significantly lower than the HFD. Correlation analysis showed that the number of bifidobacterium in intestinal and blood lipids index (TC, TQ LDL-C) were significantly negative correlation, escherichia coli number and MDA was significantly positively related to gut. Indicates that a high-fat diet may change in the gut REDOX state, destroy the environment to the survival of bacteria to influence the composition of intestinal flora, and lipid metabolism disorders. PBF and BWP have to adjust the action of gut bacteria, may thus inhibiting lipid disorders and oxidative stress of the organization.To sum up, the more difficult to digest of buckwheat protein adsorption cholic acid salt and cholesterol can promote the excretion, and regulating blood lipid metabolism and adjust the balance of intestinal flora, and increased proportion of probiotic bacteria in the gut bacteria and harmful bacteria lower proportion of lipid metabolic regulation has a role in promoting and inhibiting oxidative stress. Therefore, buckwheat protein effect on blood lipid metabolic regulation to promote bile acid salt excretion, regulating intestinal flora balance, improve pathways have a close relationship between oxidative stress.