Study On The Effect Of Buckwheat Resistant Starch On Intestinal Flora

With the improvement of people's living standards and health care awareness,diabetes,obesity,high blood pressure and other diseases increased year by year the issue has aroused people's attention.The key to solving this problem is to promote the use of high dietary fiber foods.Buckwheat resistant starch not only has the physiological function of dietary fiber,but also has good processing characteristics,has important industrial application value and market development prospect.In this paper,buckwheat starch was selected as the research object.The main conclusions are as follows:The effects of buckwheat resistant starch on intestinal microflora were studied and analyzed by gas chromatography-mass spectrometry(GC-MS)and fluorescence quantitative PCR(PCR).The effects of buckwheat starch on intestinal tract Probiotic function provides a reliable theoretical basis.(1)Buckwheat starch was used as raw material,and resistant starch was prepared by pressure heat-enzymatic hydrolysis method.The content of 32.08% buckwheat resistant starch was detected by resistance starch analysis kit.To study the effect of buckwheat resistant starch on intestinal flora by in vitro model,and to determine the representative flora and short chain fatty acid in human fecal fermentation broth.And the probiotic effect of buckwheat resistant starch was evaluated by the index of yield factor(PI).The results of plate culture showed that buckwheat resistant starch could increase the number of bifidobacteria and lactobacilli in feces and inhibit the number of Escherichia coli and Enterococcus to a certain extent.Which is more prominent is lactic acid bacteria reached 8.9×107/mL,bifidobacteria to 7.6×108/mL.Buckwheat resistant starch can increase the PI value and have the effect of prebiotics(2)The pH of the fermentation broth was determined at different time points.The results showed that the pH value of the buckwheat resistant starch group was significantly decreased except the blank group,and the lowest pH value in the high dose group was 3.73(3)The short-chain fatty acid content in the fermented broth of manure was increased by acetic acid,acetic acid,propionic acid,butyric acid and lactic acid in short-chain fatty acids by gas chromatography.The most obvious of which is butyric(4)acid and lactic acid,respectively,reached 22.28 mM and 61.03 mM.(5)The number of major intestinal flora in human feces was determined by fluorescence quantitative PCR.The results showed that the buckwheat resistant starch group had some proliferative effect on the beneficial bacteria(Bifidobacterium and Lactobacillus),and had some inhibitory effects on the growth of harmful bacteria(Enterococcus and Enterobacteriaceae and C.perfringens).Thus,Buckwheat resistant starch has a modulating effect on the number of intestinal flora.