| Background: Biomedical materials in the clinical application of orthopedic status is self-evident,the traditional titanium alloy with its light and flexible metal properties,is used as a bone defect repair material of the protagonist of clinical applications.However,its disadvantages are also obvious.Patients usually require secondary surgery to remove the biological material;the existing stress shielding has not yet been well resolved.To carry out a good combination with their own organization,and can be absorbed by the body of cost-effective bone defect material research has broad prospects.At present,biodegradable magnesium alloy is the forefront of medical metal materials in the field,which can overcome the inconvenience of re-operation of traditional implant materials such as titanium alloy and stainless steel.However,how to obtain a magnesium alloy with good mechanical compatibility and biocompatibility is a big problem to be solvedObjective: Our group used low temperature ball milling and powder metallurgy method to prepare bioactive ceramic particle reinforced magnesium matrix composites.Through the research of biocompatibility of this medical magnesium base material,in vitro culture of biological material leaching solution was carried out and implanted In vivo observation of metabolic changes in rabbits.This will further reveal the degradation behavior of Mg-based composites and the influence of degradation products on the structure of the organism,and explore the biocompatibility evaluation methods of Mg-based composites.This study will help further elucidate the mechanism of in vivo fusion and degradation of biomagnesium-based materials,and provide a theoretical basis for clinical development and application,making the new medical magnesium-based composite material a new source of future bone defect materials.Methods: To study whether BM does harm to MG63 cells,we have 3 groups including BM group,Ti group and DMSO group.Making the leach liquor of BM alloy and Ti alloy,and we also take 0.64% phenol group as positive control.We use CCK8 to detect the activities of cells proliferations in different times.Cells apoptosis in different timeswere detected by Annexin V PI double staining flow cytometry.Observating the condition off each group is done by microscope.To study whether BM does harm to the internal environment homeostasis,BM magnesium alloy and control groups of Ti alloy were made into intramedullary needle of 1 mm x 30 mm.After disinfection,asepsis operation has been done and the magnesium alloy intramedullary needle was implanted into the left femoral marrow cavity of the male New Zealand Rabbit.Each group has 6 rabbits.Weight,Urine protein,Mg,Na,can be done.To find the renal damage,HE staining of kidney is also done.CT is used to observe the detail bone condition.Results: In vitro experiments,MG63 cells in BM group and Ti group grow well.The cell frequency of BM group is nearly 85-95%.The results of CCK8 also suggest no big difference of Ti group(81.2%)and BM group(83.5%).Change of p H value changes a lot in DMSO group.In the opposite,there aren't big change of p H value in Ti group and BM group.In vivo experiment,we did't find much difference in weight,urine protein,Mg,Na,HE staining of rabbit kidney.Conclusion: Our group of new biomagnesium-based composite materials in vitro and in vivo experiments without harm to the human body,can maintain a good ecological balance in vivo,may become a new source of bone defect material. |
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